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Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)
Xu, Z.1,2; Chang, M. X.1; Qi, Z. T.1; Huang, B.1; Nie, P.1; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2009-10-15
Source PublicationVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN0165-2427
Volume131Issue:3-4Pages:268-272
AbstractThe immunoglobulin (Ig) joining (J) chain plays an important role in the formation of polymeric Igs and their transport into secretions. In the present study, the cDNA sequence of J chain has been cloned from the Chinese soft-shelled turtle (Pelodiscus sinensis) by reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence is 2347 bp in length and contains an open reading frame of 480 bp encoding 160 aa including the signal sequence. The deduced amino acid sequence has a high degree of homology with that of an already reported turtle J chain (80.7%), and of chicken (71.3%). By using real-time quantitative RT-PCR analysis, a significant up-regulation of J-chain transcripts was observed in spleen, kidney and blood of turtles injected with inactivated Aeromonas hydrophila, indicating the immune role of J chain in response to bacterial infection. (C) 2009 Elsevier B.V. All rights reserved.; The immunoglobulin (Ig) joining (J) chain plays an important role in the formation of polymeric Igs and their transport into secretions. In the present study, the cDNA sequence of J chain has been cloned from the Chinese soft-shelled turtle (Pelodiscus sinensis) by reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence is 2347 bp in length and contains an open reading frame of 480 bp encoding 160 aa including the signal sequence. The deduced amino acid sequence has a high degree of homology with that of an already reported turtle J chain (80.7%), and of chicken (71.3%). By using real-time quantitative RT-PCR analysis, a significant up-regulation of J-chain transcripts was observed in spleen, kidney and blood of turtles injected with inactivated Aeromonas hydrophila, indicating the immune role of J chain in response to bacterial infection. (C) 2009 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordJoining (j) Chain Cdna Sequence Expression Chinese Soft-shelled Turtle Pelodiscus Sinensis Bacterial Infection
Department[Xu, Z.; Chang, M. X.; Qi, Z. T.; Huang, B.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Xu, Z.] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China
Subject AreaImmunology ; Veterinary Sciences
DOI10.1016/j.vetimm.2009.04.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010] ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010] ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010] ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Veterinary Sciences
WOS SubjectImmunology ; Veterinary Sciences
WOS IDWOS:000270487400013
WOS Keyword(J)-CHAIN CDNA ; CLONING ; SEQUENCE ; GENE
Funding OrganizationNational Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010] ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010] ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010] ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7562
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Xu, Z.,Chang, M. X.,Qi, Z. T.,et al. Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2009,131(3-4):268-272.
APA Xu, Z.,Chang, M. X.,Qi, Z. T.,Huang, B.,Nie, P.,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.(2009).Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis).VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,131(3-4),268-272.
MLA Xu, Z.,et al."Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 131.3-4(2009):268-272.
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