IHB OpenIR  > 期刊论文
Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography
Wu, Xingqiang; Xiao, Bangding; Li, Renhui; Wang, Zhi; Chen, Xiaoguo; Chen, Xudong; Xiao, BD, Chinese Acad Sci, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China
2009
Source PublicationANALYTICA CHIMICA ACTA
Volume651Issue:2Pages:241-247
AbstractMicrocystins (MCs) comprise a family of more than 80 related cyclic hepatotoxic heptapeptides. Oxidation of MCs causes cleavage of the chemically unique C-20 beta-amino acid (2S, 3S, 8S, 9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda) amino to form 2-methyl-3-methoxy-4-phenylbutanoic acid (MMPB), which has been exploited to enable analysis of the entire family. In the present study, the reaction conditions (e.g. concentration of the reactants. temperature and pH) used in the production of MMPB by oxidation of cyanobacterial samples with permanganate-periodate were optimized through a series of well-controlled batch experiments. The oxidation product (MMPB) was then directly analyzed by high-performance liquid chromatography with diode array detection. The results of this study provided insight into the influence of reaction conditions on the yield of MMPB. Specifically, the optimal conditions, including a high dose of permanganate (>= 50 mM) in saturated periodate solution at ambient temperature under alkaline conditions (pH similar to 9) over 1-4 h were proposed, as indicated by a MMPB yield of greater than 85%. The technique developed here was applied to determine the total concentration of MCs in cyanobacterial bloom samples, and indicated that the MMPB technique was a highly sensitive and accurate method of quantifying total MCs. Additionally, these results will aid in development of a highly effective analytical method for detection of MMPB as an oxidation product for evaluation of total MCs in a wide range of environmental sample matrices, including natural waters, soils (sediments) and animal tissues. (C) 2009 Elsevier B.V. All rights reserved.
KeywordMicrocystin Mmpb (2-methyl-3-methoxy-4-phenylbutyric Acid) Cyanobacterial Permanganate-periodate Liquid Chromatography-diode Array Detection
Department[Wu, Xingqiang; Xiao, Bangding; Li, Renhui; Wang, Zhi; Chen, Xudong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Wu, Xingqiang; Wang, Zhi] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Chen, Xiaoguo] Wuhan Univ Technol, Wuhan 430070, Hubei, Peoples R China
Subject AreaChemistry, Analytical
Funding OrganizationNational Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304] ; National Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304] ; National Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304] ; National Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304]
Indexed BySCI
Language英语
WOS IDWOS:000270745600016
Funding OrganizationNational Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304] ; National Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304] ; National Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304] ; National Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304]
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7560
Collection期刊论文
Corresponding AuthorXiao, BD, Chinese Acad Sci, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wu, Xingqiang,Xiao, Bangding,Li, Renhui,et al. Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography[J]. ANALYTICA CHIMICA ACTA,2009,651(2):241-247.
APA Wu, Xingqiang.,Xiao, Bangding.,Li, Renhui.,Wang, Zhi.,Chen, Xiaoguo.,...&Xiao, BD, Chinese Acad Sci, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China.(2009).Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography.ANALYTICA CHIMICA ACTA,651(2),241-247.
MLA Wu, Xingqiang,et al."Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography".ANALYTICA CHIMICA ACTA 651.2(2009):241-247.
Files in This Item:
File Name/Size DocType Version Access License
Rapid quantification(471KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, Xingqiang]'s Articles
[Xiao, Bangding]'s Articles
[Li, Renhui]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Xingqiang]'s Articles
[Xiao, Bangding]'s Articles
[Li, Renhui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Xingqiang]'s Articles
[Xiao, Bangding]'s Articles
[Li, Renhui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.