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学科主题: Chemistry, Analytical
题名: Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography
作者: Wu, Xingqiang1, 2; Xiao, Bangding1; Li, Renhui1; Wang, Zhi1, 2; Chen, Xiaoguo3; Chen, Xudong1
通讯作者: Xiao, BD, Chinese Acad Sci, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China
关键词: Microcystin ; MMPB ; (2-methyl-3-methoxy-4-phenylbutyric acid) ; Cyanobacterial ; Permanganate-periodate ; Liquid chromatography-diode array detection
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2009-10-05
DOI: 10.1016/j.aca.2009.08.026
卷: 651, 期:2, 页:241-247
收录类别: SCI
文章类型: Article
部门归属: [Wu, Xingqiang; Xiao, Bangding; Li, Renhui; Wang, Zhi; Chen, Xudong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Wu, Xingqiang; Wang, Zhi] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Chen, Xiaoguo] Wuhan Univ Technol, Wuhan 430070, Hubei, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences
资助者: National Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304]
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
摘要: Microcystins (MCs) comprise a family of more than 80 related cyclic hepatotoxic heptapeptides. Oxidation of MCs causes cleavage of the chemically unique C-20 beta-amino acid (2S, 3S, 8S, 9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda) amino to form 2-methyl-3-methoxy-4-phenylbutanoic acid (MMPB), which has been exploited to enable analysis of the entire family. In the present study, the reaction conditions (e.g. concentration of the reactants. temperature and pH) used in the production of MMPB by oxidation of cyanobacterial samples with permanganate-periodate were optimized through a series of well-controlled batch experiments. The oxidation product (MMPB) was then directly analyzed by high-performance liquid chromatography with diode array detection. The results of this study provided insight into the influence of reaction conditions on the yield of MMPB. Specifically, the optimal conditions, including a high dose of permanganate (>= 50 mM) in saturated periodate solution at ambient temperature under alkaline conditions (pH similar to 9) over 1-4 h were proposed, as indicated by a MMPB yield of greater than 85%. The technique developed here was applied to determine the total concentration of MCs in cyanobacterial bloom samples, and indicated that the MMPB technique was a highly sensitive and accurate method of quantifying total MCs. Additionally, these results will aid in development of a highly effective analytical method for detection of MMPB as an oxidation product for evaluation of total MCs in a wide range of environmental sample matrices, including natural waters, soils (sediments) and animal tissues. (C) 2009 Elsevier B.V. All rights reserved.
英文摘要: Microcystins (MCs) comprise a family of more than 80 related cyclic hepatotoxic heptapeptides. Oxidation of MCs causes cleavage of the chemically unique C(20) beta-amino acid (2S, 3S, 8S, 9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda) amino to form 2-methyl-3-methoxy-4-phenylbutanoic acid (MMPB), which has been exploited to enable analysis of the entire family. In the present study, the reaction conditions (e.g. concentration of the reactants. temperature and pH) used in the production of MMPB by oxidation of cyanobacterial samples with permanganate-periodate were optimized through a series of well-controlled batch experiments. The oxidation product (MMPB) was then directly analyzed by high-performance liquid chromatography with diode array detection. The results of this study provided insight into the influence of reaction conditions on the yield of MMPB. Specifically, the optimal conditions, including a high dose of permanganate (>= 50 mM) in saturated periodate solution at ambient temperature under alkaline conditions (pH similar to 9) over 1-4 h were proposed, as indicated by a MMPB yield of greater than 85%. The technique developed here was applied to determine the total concentration of MCs in cyanobacterial bloom samples, and indicated that the MMPB technique was a highly sensitive and accurate method of quantifying total MCs. Additionally, these results will aid in development of a highly effective analytical method for detection of MMPB as an oxidation product for evaluation of total MCs in a wide range of environmental sample matrices, including natural waters, soils (sediments) and animal tissues. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: PROTEIN PHOSPHATASE INHIBITION ; GREEN-ALGAE CYANOBACTERIA ; PEPTIDE TOXINS ; WATER ; HEPATOTOXINS ; BIOACCUMULATION ; EXTRACTION ; AERUGINOSA ; TOXICITY ; EXPOSURE
语种: 英语
WOS记录号: WOS:000270745600016
ISSN号: 0003-2670
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7560
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Wuhan Univ Technol, Wuhan 430070, Hubei, Peoples R China

Recommended Citation:
Wu, Xingqiang; Xiao, Bangding; Li, Renhui; Wang, Zhi; Chen, Xiaoguo; Chen, Xudong.Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography,ANALYTICA CHIMICA ACTA,2009,651(2):241-247
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