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PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status
Wu, Li1,2; Yu, Yuhe1; Zhang, Tanglin1; Feng, Weisong1; Zhang, Xiang1,2; Li, Wei1,2; Wu, L, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009
Source PublicationINTERNATIONAL REVIEW OF HYDROBIOLOGY
ISSN1434-2944
Volume94Issue:5Pages:528-541
AbstractPlankton communities in eight lakes of different trophic status near Yangtze, China were characterized by using denatured gradient gel electrophoresis (DGGE). Various water quality parameters were also measured at each collection site. Following extraction of DNA from plankton communities, 16S rRNA and 18S rRNA genes were amplified with specific primers for prokaryotes and eukaryotes, respectively; DNA profiles were developed by DGGE. The plankton community of each lake had its own distinct DNA profile. The total number of bands identified at 34 sampling stations ranged from 37 to 111. Both prokaryotes and eukaryotes displayed complex fingerprints composed of a large number of bands: 16 to 59 bands were obtained with the prokaryotic primer set; 21 to 52 bands for the eukaryotic primer set. The DGGE-patterns were analyzed in relation to water quality parameters by canonical correspondence analysis (CCA). Temperature, pH, alkalinity, and the concentration of COD, TP and TN were strongly correlated with the DGGE patterns. The parameters that demonstrated a strong correlation to the DGGE fingerprints of the plankton community differed among lakes, suggesting that differences in the DGGE fingerprints were due mainly to lake trophic status. Results of the present study suggest that PCR-DGGE fingerprinting is an effective and precise method of identifying changes to plankton community composition, and therefore could be a useful ecological tool for monitoring the response of aquatic ecosystems to environmental perturbations.; Plankton communities in eight lakes of different trophic status near Yangtze, China were characterized by using denatured gradient gel electrophoresis (DGGE). Various water quality parameters were also measured at each collection site. Following extraction of DNA from plankton communities, 16S rRNA and 18S rRNA genes were amplified with specific primers for prokaryotes and eukaryotes, respectively; DNA profiles were developed by DGGE. The plankton community of each lake had its own distinct DNA profile. The total number of bands identified at 34 sampling stations ranged from 37 to 111. Both prokaryotes and eukaryotes displayed complex fingerprints composed of a large number of bands: 16 to 59 bands were obtained with the prokaryotic primer set; 21 to 52 bands for the eukaryotic primer set. The DGGE-patterns were analyzed in relation to water quality parameters by canonical correspondence analysis (CCA). Temperature, pH, alkalinity, and the concentration of COD, TP and TN were strongly correlated with the DGGE patterns. The parameters that demonstrated a strong correlation to the DGGE fingerprints of the plankton community differed among lakes, suggesting that differences in the DGGE fingerprints were due mainly to lake trophic status. Results of the present study suggest that PCR-DGGE fingerprinting is an effective and precise method of identifying changes to plankton community composition, and therefore could be a useful ecological tool for monitoring the response of aquatic ecosystems to environmental perturbations.
SubtypeArticle
KeywordFreshwater Plankton Community Dna Analysis 16s Rrna 18s Rrna Water Quality
Department[Wu, Li; Yu, Yuhe; Zhang, Tanglin; Feng, Weisong; Zhang, Xiang; Li, Wei] Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Wu, Li; Zhang, Xiang; Li, Wei] Hefei Normal Univ, Dept Life Sci, Hefei 230061, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaMarine & Freshwater Biology
DOI10.1002/iroh.200911129
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMajor State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016] ; Major State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016] ; Major State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016] ; Major State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000271120200002
WOS KeywordGRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; ENRICHED SEAWATER MESOCOSMS ; BACTERIAL COMMUNITY ; MICROBIAL COMMUNITY ; GENETIC DIVERSITY ; FRESH-WATER ; ENVIRONMENTAL-FACTORS ; MOLECULAR TECHNIQUES ; SPECIES RICHNESS
Funding OrganizationMajor State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016] ; Major State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016] ; Major State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016] ; Major State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016]
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7536
Collection期刊论文
Corresponding AuthorWu, L, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Hefei Normal Univ, Dept Life Sci, Hefei 230061, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wu, Li,Yu, Yuhe,Zhang, Tanglin,et al. PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status[J]. INTERNATIONAL REVIEW OF HYDROBIOLOGY,2009,94(5):528-541.
APA Wu, Li.,Yu, Yuhe.,Zhang, Tanglin.,Feng, Weisong.,Zhang, Xiang.,...&Wu, L, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status.INTERNATIONAL REVIEW OF HYDROBIOLOGY,94(5),528-541.
MLA Wu, Li,et al."PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status".INTERNATIONAL REVIEW OF HYDROBIOLOGY 94.5(2009):528-541.
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