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学科主题: Marine & Freshwater Biology
题名: PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status
作者: Wu, Li1, 2; Yu, Yuhe1; Zhang, Tanglin1; Feng, Weisong1; Zhang, Xiang1, 2; Li, Wei1, 2
通讯作者: Wu, L, Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: freshwater plankton ; community DNA analysis ; 16S rRNA ; 18S rRNA ; water quality
刊名: INTERNATIONAL REVIEW OF HYDROBIOLOGY
发表日期: 2009
DOI: 10.1002/iroh.200911129
卷: 94, 期:5, 页:528-541
收录类别: SCI
文章类型: Article
部门归属: [Wu, Li; Yu, Yuhe; Zhang, Tanglin; Feng, Weisong; Zhang, Xiang; Li, Wei] Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Wu, Li; Zhang, Xiang; Li, Wei] Hefei Normal Univ, Dept Life Sci, Hefei 230061, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Major State Basic Research Development Program of China [2007CB 109205]; National Natural Science Foundation of China [30770298]; Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnology [20081713016]
类目[WOS]: Marine & Freshwater Biology
研究领域[WOS]: Marine & Freshwater Biology
摘要: Plankton communities in eight lakes of different trophic status near Yangtze, China were characterized by using denatured gradient gel electrophoresis (DGGE). Various water quality parameters were also measured at each collection site. Following extraction of DNA from plankton communities, 16S rRNA and 18S rRNA genes were amplified with specific primers for prokaryotes and eukaryotes, respectively; DNA profiles were developed by DGGE. The plankton community of each lake had its own distinct DNA profile. The total number of bands identified at 34 sampling stations ranged from 37 to 111. Both prokaryotes and eukaryotes displayed complex fingerprints composed of a large number of bands: 16 to 59 bands were obtained with the prokaryotic primer set; 21 to 52 bands for the eukaryotic primer set. The DGGE-patterns were analyzed in relation to water quality parameters by canonical correspondence analysis (CCA). Temperature, pH, alkalinity, and the concentration of COD, TP and TN were strongly correlated with the DGGE patterns. The parameters that demonstrated a strong correlation to the DGGE fingerprints of the plankton community differed among lakes, suggesting that differences in the DGGE fingerprints were due mainly to lake trophic status. Results of the present study suggest that PCR-DGGE fingerprinting is an effective and precise method of identifying changes to plankton community composition, and therefore could be a useful ecological tool for monitoring the response of aquatic ecosystems to environmental perturbations.
英文摘要: Plankton communities in eight lakes of different trophic status near Yangtze, China were characterized by using denatured gradient gel electrophoresis (DGGE). Various water quality parameters were also measured at each collection site. Following extraction of DNA from plankton communities, 16S rRNA and 18S rRNA genes were amplified with specific primers for prokaryotes and eukaryotes, respectively; DNA profiles were developed by DGGE. The plankton community of each lake had its own distinct DNA profile. The total number of bands identified at 34 sampling stations ranged from 37 to 111. Both prokaryotes and eukaryotes displayed complex fingerprints composed of a large number of bands: 16 to 59 bands were obtained with the prokaryotic primer set; 21 to 52 bands for the eukaryotic primer set. The DGGE-patterns were analyzed in relation to water quality parameters by canonical correspondence analysis (CCA). Temperature, pH, alkalinity, and the concentration of COD, TP and TN were strongly correlated with the DGGE patterns. The parameters that demonstrated a strong correlation to the DGGE fingerprints of the plankton community differed among lakes, suggesting that differences in the DGGE fingerprints were due mainly to lake trophic status. Results of the present study suggest that PCR-DGGE fingerprinting is an effective and precise method of identifying changes to plankton community composition, and therefore could be a useful ecological tool for monitoring the response of aquatic ecosystems to environmental perturbations.
关键词[WOS]: GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; ENRICHED SEAWATER MESOCOSMS ; BACTERIAL COMMUNITY ; MICROBIAL COMMUNITY ; GENETIC DIVERSITY ; FRESH-WATER ; ENVIRONMENTAL-FACTORS ; MOLECULAR TECHNIQUES ; SPECIES RICHNESS
语种: 英语
WOS记录号: WOS:000271120200002
ISSN号: 1434-2944
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7536
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Hefei Normal Univ, Dept Life Sci, Hefei 230061, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Wu, Li; Yu, Yuhe; Zhang, Tanglin; Feng, Weisong; Zhang, Xiang; Li, Wei.PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status,INTERNATIONAL REVIEW OF HYDROBIOLOGY,2009,94(5):528-541
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