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学科主题: Chemistry ; Analytical
题名: Detection of microcystins in environmental samples using surface plasmon resonance biosensor
作者: Hu, Chenlin1, 2; Gan, Nanqin1; Chen, Yuanyuan3; Bi, Lijun3; Zhang, Xianen3; Song, Lirong1
通讯作者: Song, LR, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
关键词: Microcystins ; Surface plasmon resonance (SPR) ; Biosensor ; ELISA
刊名: TALANTA
发表日期: 2009-11-15
DOI: 10.1016/j.talanta.2009.06.044
卷: 80, 期:1, 页:407-410
收录类别: SCI
文章类型: Article
部门归属: [Hu, Chenlin; Gan, Nanqin; Song, Lirong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Hu, Chenlin] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Chen, Yuanyuan; Bi, Lijun; Zhang, Xianen] Chinese Acad Sci, Inst Biophys, Analyt Biotechnol State Key Lab Macromol, Beijing 100101, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences
资助者: National Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1]
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
摘要: An indirect inhibitive surface plasmon resonance (SPR) immunoassay was developed for the microcystins (MCs) detection. The bioconjugate of MC-LR and bovine serum albumin (BSA) was immobilized on a CM5 sensor chip. A serial premixture of MC-LR standards (or samples) and monoclonal antibody (mAb) were injected over the functional sensor surface, and the subsequent specific immunoreaction was monitored on the BIAcore 3000 biosensor and generated a signal with an increasing intensity in response to the decreasing MCs concentration. The developed SPR immunoassay has a wide quantitative range in 1-100 mu g L-1. Although not as sensitive as conventional enzyme-linked immunosorbent assay (ELISA), the SPR biosensor offered unique advantages: (I) the sensor chip could be reusable without any significant loss in its binding activity after 50 assay-regeneration cycles, (2) one single assay could be accomplished in 50 min (including 30-min preincubation and 20-min BIAcore analysis), and (3) this method did not require multiple steps. The SPR biosensor was also used to detect MCs in environmental samples, and the results compared well with those obtained by ELISA. We conclude that the SPR biosensor offers outstanding advantages for the MCs detection and may be further developed as a field-portable sensor for real-time monitoring of MCs on site in the near future. (C) 2009 Published by Elsevier B.V.
英文摘要: An indirect inhibitive surface plasmon resonance (SPR) immunoassay was developed for the microcystins (MCs) detection. The bioconjugate of MC-LR and bovine serum albumin (BSA) was immobilized on a CM5 sensor chip. A serial premixture of MC-LR standards (or samples) and monoclonal antibody (mAb) were injected over the functional sensor surface, and the subsequent specific immunoreaction was monitored on the BIAcore 3000 biosensor and generated a signal with an increasing intensity in response to the decreasing MCs concentration. The developed SPR immunoassay has a wide quantitative range in 1-100 mu g L(-1). Although not as sensitive as conventional enzyme-linked immunosorbent assay (ELISA), the SPR biosensor offered unique advantages: (I) the sensor chip could be reusable without any significant loss in its binding activity after 50 assay-regeneration cycles, (2) one single assay could be accomplished in 50 min (including 30-min preincubation and 20-min BIAcore analysis), and (3) this method did not require multiple steps. The SPR biosensor was also used to detect MCs in environmental samples, and the results compared well with those obtained by ELISA. We conclude that the SPR biosensor offers outstanding advantages for the MCs detection and may be further developed as a field-portable sensor for real-time monitoring of MCs on site in the near future. (C) 2009 Published by Elsevier B.V.
关键词[WOS]: SMALL MOLECULES ; IMMUNOASSAY ; IMMUNOSENSORS ; LR
语种: 英语
WOS记录号: WOS:000271055700060
ISSN号: 0039-9140
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7530
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Analyt Biotechnol State Key Lab Macromol, Beijing 100101, Peoples R China

Recommended Citation:
Hu, Chenlin; Gan, Nanqin; Chen, Yuanyuan; Bi, Lijun; Zhang, Xianen; Song, Lirong.Detection of microcystins in environmental samples using surface plasmon resonance biosensor,TALANTA,2009,80(1):407-410
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