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Detection of microcystins in environmental samples using surface plasmon resonance biosensor
Hu, Chenlin1,2; Gan, Nanqin1; Chen, Yuanyuan3; Bi, Lijun3; Zhang, Xianen3; Song, Lirong1; Song, LR, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
2009-11-15
Source PublicationTALANTA
ISSN0039-9140
Volume80Issue:1Pages:407-410
AbstractAn indirect inhibitive surface plasmon resonance (SPR) immunoassay was developed for the microcystins (MCs) detection. The bioconjugate of MC-LR and bovine serum albumin (BSA) was immobilized on a CM5 sensor chip. A serial premixture of MC-LR standards (or samples) and monoclonal antibody (mAb) were injected over the functional sensor surface, and the subsequent specific immunoreaction was monitored on the BIAcore 3000 biosensor and generated a signal with an increasing intensity in response to the decreasing MCs concentration. The developed SPR immunoassay has a wide quantitative range in 1-100 mu g L-1. Although not as sensitive as conventional enzyme-linked immunosorbent assay (ELISA), the SPR biosensor offered unique advantages: (I) the sensor chip could be reusable without any significant loss in its binding activity after 50 assay-regeneration cycles, (2) one single assay could be accomplished in 50 min (including 30-min preincubation and 20-min BIAcore analysis), and (3) this method did not require multiple steps. The SPR biosensor was also used to detect MCs in environmental samples, and the results compared well with those obtained by ELISA. We conclude that the SPR biosensor offers outstanding advantages for the MCs detection and may be further developed as a field-portable sensor for real-time monitoring of MCs on site in the near future. (C) 2009 Published by Elsevier B.V.; An indirect inhibitive surface plasmon resonance (SPR) immunoassay was developed for the microcystins (MCs) detection. The bioconjugate of MC-LR and bovine serum albumin (BSA) was immobilized on a CM5 sensor chip. A serial premixture of MC-LR standards (or samples) and monoclonal antibody (mAb) were injected over the functional sensor surface, and the subsequent specific immunoreaction was monitored on the BIAcore 3000 biosensor and generated a signal with an increasing intensity in response to the decreasing MCs concentration. The developed SPR immunoassay has a wide quantitative range in 1-100 mu g L(-1). Although not as sensitive as conventional enzyme-linked immunosorbent assay (ELISA), the SPR biosensor offered unique advantages: (I) the sensor chip could be reusable without any significant loss in its binding activity after 50 assay-regeneration cycles, (2) one single assay could be accomplished in 50 min (including 30-min preincubation and 20-min BIAcore analysis), and (3) this method did not require multiple steps. The SPR biosensor was also used to detect MCs in environmental samples, and the results compared well with those obtained by ELISA. We conclude that the SPR biosensor offers outstanding advantages for the MCs detection and may be further developed as a field-portable sensor for real-time monitoring of MCs on site in the near future. (C) 2009 Published by Elsevier B.V.
SubtypeArticle
KeywordMicrocystins Surface Plasmon Resonance (Spr) Biosensor Elisa
Department[Hu, Chenlin; Gan, Nanqin; Song, Lirong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Hu, Chenlin] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Chen, Yuanyuan; Bi, Lijun; Zhang, Xianen] Chinese Acad Sci, Inst Biophys, Analyt Biotechnol State Key Lab Macromol, Beijing 100101, Peoples R China
Subject AreaChemistry ; Analytical
DOI10.1016/j.talanta.2009.06.044
WOS HeadingsScience & Technology ; Physical Sciences
Funding OrganizationNational Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1] ; National Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1] ; National Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1] ; National Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1]
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000271055700060
WOS KeywordSMALL MOLECULES ; IMMUNOASSAY ; IMMUNOSENSORS ; LR
Funding OrganizationNational Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1] ; National Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1] ; National Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1] ; National Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1]
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7530
Collection期刊论文
Corresponding AuthorSong, LR, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Analyt Biotechnol State Key Lab Macromol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Hu, Chenlin,Gan, Nanqin,Chen, Yuanyuan,et al. Detection of microcystins in environmental samples using surface plasmon resonance biosensor[J]. TALANTA,2009,80(1):407-410.
APA Hu, Chenlin.,Gan, Nanqin.,Chen, Yuanyuan.,Bi, Lijun.,Zhang, Xianen.,...&Song, LR, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Donghu Nanlu 7, Wuhan 430072, Peoples R China.(2009).Detection of microcystins in environmental samples using surface plasmon resonance biosensor.TALANTA,80(1),407-410.
MLA Hu, Chenlin,et al."Detection of microcystins in environmental samples using surface plasmon resonance biosensor".TALANTA 80.1(2009):407-410.
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