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学科主题: Multidisciplinary Sciences
题名: Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio
作者: Wang, Da1; Mao, Huiling2; Peng, Jinxia1; Li, Xiyin1; Zhou, Li1; Gui, Jianfang1
通讯作者: Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: Gibel carp (Carassius auratus gibelio) ; Gynogenesis ; Male-biased mutant family ; Male-specific SCAR marker (MSM)
刊名: PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL
发表日期: 2009-11-10
DOI: 10.1016/j.pnsc.2009.04.008
卷: 19, 期:11, 页:1537-1544
收录类别: SCI
文章类型: Article
部门归属: [Wang, Da; Peng, Jinxia; Li, Xiyin; Zhou, Li; Gui, Jianfang] Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Mao, Huiling] Nanchang Univ, Sch Life Sci, Nanchang 330031, Peoples R China
WOS标题词: Science & Technology ; Technology
资助者: National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072]
类目[WOS]: Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]: Materials Science ; Science & Technology - Other Topics
摘要: Gibel carp ( Carassius auratus gibelio) is a uniquely gynogenetic species with a minor ratio of males in natural habitats, but its male origin and sex determination mechanisms have been unknown. In this study, a male-biased mutant family was discovered from the gynogenetic gibel carp, and a male-specific SCAR marker was identified from the mutant family. Normal spermatogenesis was observed in the male testes by immuno. fluorescence histochemistry. Nearly identical AFLP profiles were observed between males and females, but a male-specific 86 bp AFLP fragment was screened by sex-pool bulked segregant analysis and individual screening. Based on the male-specific AFLP fragment, a total of 579 bp sequences were cloned by genome walking. Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要: Gibel carp ( Carassius auratus gibelio) is a uniquely gynogenetic species with a minor ratio of males in natural habitats, but its male origin and sex determination mechanisms have been unknown. In this study, a male-biased mutant family was discovered from the gynogenetic gibel carp, and a male-specific SCAR marker was identified from the mutant family. Normal spermatogenesis was observed in the male testes by immuno. fluorescence histochemistry. Nearly identical AFLP profiles were observed between males and females, but a male-specific 86 bp AFLP fragment was screened by sex-pool bulked segregant analysis and individual screening. Based on the male-specific AFLP fragment, a total of 579 bp sequences were cloned by genome walking. Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
关键词[WOS]: SEX-DETERMINING REGION ; Y-CHROMOSOME ; FISH ; BLOCH ; GENE ; DIFFERENTIATION ; DNA ; REPRODUCTION ; EVOLUTION ; PROTEIN
语种: 英语
WOS记录号: WOS:000271340100011
ISSN号: 1002-0071
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7510
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Nanchang Univ, Sch Life Sci, Nanchang 330031, Peoples R China

Recommended Citation:
Wang, Da; Mao, Huiling; Peng, Jinxia; Li, Xiyin; Zhou, Li; Gui, Jianfang.Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio,PROGRESS IN NATURAL SCIENCE,2009,19(11):1537-1544
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