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Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio
Wang, Da; Mao, Huiling; Peng, Jinxia; Li, Xiyin; Zhou, Li; Gui, Jianfang; Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009
Source PublicationPROGRESS IN NATURAL SCIENCE
Volume19Issue:11Pages:1537-1544
AbstractGibel carp ( Carassius auratus gibelio) is a uniquely gynogenetic species with a minor ratio of males in natural habitats, but its male origin and sex determination mechanisms have been unknown. In this study, a male-biased mutant family was discovered from the gynogenetic gibel carp, and a male-specific SCAR marker was identified from the mutant family. Normal spermatogenesis was observed in the male testes by immuno. fluorescence histochemistry. Nearly identical AFLP profiles were observed between males and females, but a male-specific 86 bp AFLP fragment was screened by sex-pool bulked segregant analysis and individual screening. Based on the male-specific AFLP fragment, a total of 579 bp sequences were cloned by genome walking. Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
KeywordGibel Carp (Carassius Auratus Gibelio) Gynogenesis Male-biased Mutant Family Male-specific Scar Marker (Msm)
Department[Wang, Da; Peng, Jinxia; Li, Xiyin; Zhou, Li; Gui, Jianfang] Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Mao, Huiling] Nanchang Univ, Sch Life Sci, Nanchang 330031, Peoples R China
Subject AreaMultidisciplinary Sciences
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072]
Indexed BySCI
Language英语
WOS IDWOS:000271340100011
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072] ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072]
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Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7510
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Da,Mao, Huiling,Peng, Jinxia,et al. Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio[J]. PROGRESS IN NATURAL SCIENCE,2009,19(11):1537-1544.
APA Wang, Da.,Mao, Huiling.,Peng, Jinxia.,Li, Xiyin.,Zhou, Li.,...&Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio.PROGRESS IN NATURAL SCIENCE,19(11),1537-1544.
MLA Wang, Da,et al."Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio".PROGRESS IN NATURAL SCIENCE 19.11(2009):1537-1544.
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