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学科主题: Multidisciplinary Sciences
题名: Outer membrane proteins induced by iron deficiency in Anabaena sp PCC 7120
作者: Dong, Yanling1; Xu, Xudong1
通讯作者: Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Anabaena ; Outer membrane ; Siderophore receptors ; Iron deficiency
刊名: PROGRESS IN NATURAL SCIENCE
发表日期: 2009-11-10
DOI: 10.1016/j.pnsc.2009.02.009
卷: 19, 期:11, 页:1477-1483
收录类别: SCI
文章类型: Article
部门归属: [Dong, Yanling; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Technology
资助者: State Key Basic Research Development Program [2008CB418001]; National Natural Science Foundation of China [30825003]
类目[WOS]: Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]: Materials Science ; Science & Technology - Other Topics
摘要: Iron deficiency can induce cyanobacteria to synthesize siderophore receptor proteins on the outer membrane to enhance the uptake of iron. In this study, an outer membrane of high purity was prepared from Anabaena sp. PCC 7120 based on aqueous polymer two-phase partitioning and discontinuous sucrose density ultra-centrifugation, and the induction of outer membrane proteins by iron deficiency was investigated using 2-D gel electrophoresis. At least. five outer membrane proteins were newly synthesized or significantly up-regulated in cells transferred to iron-deficient conditions, which were all identified to be siderophore receptor proteins according to MALDI-TOF-MS analyses. Bacterial luciferase reporter genes luxAB were employed to monitor the transcription of the encoding genes. The genes were induced by iron deficiency at the transcriptional level in different responsive modes. Luciferase activity expressed from an iron-regulated promoter may be used as a bioreporter for utilizable iron in natural water samples. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要: Iron deficiency can induce cyanobacteria to synthesize siderophore receptor proteins on the outer membrane to enhance the uptake of iron. In this study, an outer membrane of high purity was prepared from Anabaena sp. PCC 7120 based on aqueous polymer two-phase partitioning and discontinuous sucrose density ultra-centrifugation, and the induction of outer membrane proteins by iron deficiency was investigated using 2-D gel electrophoresis. At least. five outer membrane proteins were newly synthesized or significantly up-regulated in cells transferred to iron-deficient conditions, which were all identified to be siderophore receptor proteins according to MALDI-TOF-MS analyses. Bacterial luciferase reporter genes luxAB were employed to monitor the transcription of the encoding genes. The genes were induced by iron deficiency at the transcriptional level in different responsive modes. Luciferase activity expressed from an iron-regulated promoter may be used as a bioreporter for utilizable iron in natural water samples. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
关键词[WOS]: SP STRAIN PCC-7120 ; SYNECHOCYSTIS-SP PCC-6803 ; CYANOBACTERIUM ANABAENA ; OXIDATIVE STRESS ; SIDEROPHORE ; GENES ; SCHIZOKINEN ; EXPRESSION ; TRANSPORT ; NTCA
语种: 英语
WOS记录号: WOS:000271340100003
ISSN号: 1002-0071
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7508
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Dong, Yanling; Xu, Xudong.Outer membrane proteins induced by iron deficiency in Anabaena sp PCC 7120,PROGRESS IN NATURAL SCIENCE,2009,19(11):1477-1483
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