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Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations
Wang, D.1; Mao, H. -L.2; Chen, H. -X.1; Liu, H. -Q.3,4; Gui, J. -F.1; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China
2009-12-01
Source PublicationANIMAL GENETICS
ISSN0268-9146
Volume40Issue:6Pages:978-981
AbstractP>Sex controls have been performed in some farmed fish species because of significant growth differences between females and males. In yellow catfish (Pelteobagrus fulvidraco), adult males are three times larger than female adults. In this study, six Y- and X-linked amplified fragment length polymorphism fragments were screened by sex-genotype pool bulked segregant analysis and individual screening. Interestingly, sequence analysis identified two pairs of allelic genes, Pf33 and Pf62. Furthermore, the cloned flanking sequences revealed several Y- and X-specific polymorphisms, and four Y-linked or X-linked sequence characterized amplified region (SCAR) primer pairs were designed and converted into Y- and X-linked SCAR markers. Consequently, these markers were successfully used to identify genetic sex and YY super-males, and applied to all-male population production. Thus, we developed a novel and simple technique to help commercial production of YY super-males and all-male populations in the yellow catfish.; P>Sex controls have been performed in some farmed fish species because of significant growth differences between females and males. In yellow catfish (Pelteobagrus fulvidraco), adult males are three times larger than female adults. In this study, six Y- and X-linked amplified fragment length polymorphism fragments were screened by sex-genotype pool bulked segregant analysis and individual screening. Interestingly, sequence analysis identified two pairs of allelic genes, Pf33 and Pf62. Furthermore, the cloned flanking sequences revealed several Y- and X-specific polymorphisms, and four Y-linked or X-linked sequence characterized amplified region (SCAR) primer pairs were designed and converted into Y- and X-linked SCAR markers. Consequently, these markers were successfully used to identify genetic sex and YY super-males, and applied to all-male population production. Thus, we developed a novel and simple technique to help commercial production of YY super-males and all-male populations in the yellow catfish.
SubtypeArticle
KeywordAflp All-male Pelteobagrus Fulvidraco X-linked Scar Marker Y-linked Scar Marker Yy Super-male
Department[Wang, D.; Chen, H. -X.; Gui, J. -F.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China; [Mao, H. -L.] Nanchang Univ, Sch Life Sci, Nanchang 330031, Peoples R China; [Liu, H. -Q.] Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China; [Liu, H. -Q.] Chinese Acad Sci, Wuhan 430079, Peoples R China
Subject AreaAgriculture, Dairy & Animal Science ; Genetics & Heredity
DOI10.1111/j.1365-2052.2009.01941.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01] ; National Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01] ; National Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01] ; National Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01]
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Genetics & Heredity
WOS SubjectAgriculture, Dairy & Animal Science ; Genetics & Heredity
WOS IDWOS:000271519700025
WOS KeywordBULKED SEGREGANT ANALYSIS ; FULVIDRACO RICHARDSON ; RAINBOW-TROUT ; SEX ; IDENTIFICATION ; TILAPIA ; FEMALE ; AFLP ; GROWTH ; CARP
Funding OrganizationNational Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01] ; National Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01] ; National Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01] ; National Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7506
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China
2.Nanchang Univ, Sch Life Sci, Nanchang 330031, Peoples R China
3.Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China
4.Chinese Acad Sci, Wuhan 430079, Peoples R China
Recommended Citation
GB/T 7714
Wang, D.,Mao, H. -L.,Chen, H. -X.,et al. Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations[J]. ANIMAL GENETICS,2009,40(6):978-981.
APA Wang, D.,Mao, H. -L.,Chen, H. -X.,Liu, H. -Q.,Gui, J. -F.,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China.(2009).Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations.ANIMAL GENETICS,40(6),978-981.
MLA Wang, D.,et al."Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations".ANIMAL GENETICS 40.6(2009):978-981.
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