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Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements
Chen, Chun-Hong1,2,3; Sun, Yong-Hua1; Pei, De-Sheng1; Zhu, Zuo-Yan1,2,3; Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2009-11-01
Source PublicationDEVELOPMENTAL DYNAMICS
ISSN1058-8388
Volume238Issue:11Pages:2850-2859
AbstractLarge tumor suppressor (Lats) is a Ser/Thr kinase, and it presents an important function in tumor suppression. lats was originally identified in Drosophila and recently in mammals. In mammals, it contains two homologues, lats1 and lats2. In the present study, lats1 and lats2 were characterized from zebrafish (Danio rerio), which is the first report of lats in a nonmammalian vertebrate. The primary structure, genomic organization, and phylogenesis of lats from different species were studied, and the results suggest that lats1 is the direct descendant of invertebrate lats, whereas lats2 is formed by genome duplication. In zebrafish, both lats genes are maternally expressed, while they show distinctly different expression profiles during gastrulation. lats1 is almost ubiquitously expressed through development, and lats2 is more prominently expressed in the non-neural ectoderm region of zebrafish gastrula. Most intriguingly, as revealed by cell tracing and gene expression analysis, morpholino-mediated knockdown of either lats1 or lats2 led to obvious defects of cell migration in gastrulation, indicating the functional significance of lats in gastrulation movements. Developmental Dynamics 238:28502859, 2009. (C) 2009 Wiley-Liss, Inc.; Large tumor suppressor (Lats) is a Ser/Thr kinase, and it presents an important function in tumor suppression. lats was originally identified in Drosophila and recently in mammals. In mammals, it contains two homologues, lats1 and lats2. In the present study, lats1 and lats2 were characterized from zebrafish (Danio rerio), which is the first report of lats in a nonmammalian vertebrate. The primary structure, genomic organization, and phylogenesis of lats from different species were studied, and the results suggest that lats1 is the direct descendant of invertebrate lats, whereas lats2 is formed by genome duplication. In zebrafish, both lats genes are maternally expressed, while they show distinctly different expression profiles during gastrulation. lats1 is almost ubiquitously expressed through development, and lats2 is more prominently expressed in the non-neural ectoderm region of zebrafish gastrula. Most intriguingly, as revealed by cell tracing and gene expression analysis, morpholino-mediated knockdown of either lats1 or lats2 led to obvious defects of cell migration in gastrulation, indicating the functional significance of lats in gastrulation movements. Developmental Dynamics 238:28502859, 2009. (C) 2009 Wiley-Liss, Inc.
SubtypeArticle
KeywordZebrafish Lats Phylogenetic Analysis Spatiotemporal Expression Covergence Extention Movement
Department[Chen, Chun-Hong; Sun, Yong-Hua; Pei, De-Sheng; Zhu, Zuo-Yan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Chen, Chun-Hong; Zhu, Zuo-Yan] Wuhan Univ, Coll Life Sci, Dept Genet, Wuhan 430072, Peoples R China; [Chen, Chun-Hong; Zhu, Zuo-Yan] Wuhan Univ, Coll Life Sci, Ctr Dev Biol, Wuhan 430072, Peoples R China
Subject AreaAnatomy & Morphology ; Developmental Biology
DOI10.1002/dvdy.22105
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationState Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100]
Indexed BySCI
Language英语
WOS Research AreaAnatomy & Morphology ; Developmental Biology
WOS SubjectAnatomy & Morphology ; Developmental Biology
WOS IDWOS:000271548800012
WOS KeywordDROSOPHILA TUMOR-SUPPRESSOR ; CELL MOVEMENTS ; PROTEIN-KINASE ; EXTENSION ; GENE ; CONVERGENCE ; WARTS/LATS ; REGULATOR ; POLARITY ; HOMOLOG
Funding OrganizationState Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100] ; State Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7502
Collection期刊论文
Corresponding AuthorSun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Wuhan Univ, Coll Life Sci, Dept Genet, Wuhan 430072, Peoples R China
3.Wuhan Univ, Coll Life Sci, Ctr Dev Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Chun-Hong,Sun, Yong-Hua,Pei, De-Sheng,et al. Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements[J]. DEVELOPMENTAL DYNAMICS,2009,238(11):2850-2859.
APA Chen, Chun-Hong,Sun, Yong-Hua,Pei, De-Sheng,Zhu, Zuo-Yan,&Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2009).Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements.DEVELOPMENTAL DYNAMICS,238(11),2850-2859.
MLA Chen, Chun-Hong,et al."Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements".DEVELOPMENTAL DYNAMICS 238.11(2009):2850-2859.
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