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Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis
Wei, Yanhong1; Shi, Xiongjie2; Zhang, Hongxia1; Wang, Jianshe1; Zhou, Bingsheng2; Dai, Jiayin1; Dai, JY, Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China
2009-10-19
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume95Issue:1Pages:27-36
AbstractPolyfluorinated and perfluorinated compounds (PFCs) are used in numerous commercial products and have been ubiquitously detected in the environment as well as in the blood of humans and wildlife. To assess the combined effects caused by PFCs in mixtures, gene expression profiles were generated using a custom cDNA microarray to detect changes in primary cultured hepatocytes of rare minnows exposed to six individual PFCs (perfluorooctanoic acid, perfluorononanoic acid, perfluorodecanoic acid, perfluorododecanoic acid, perfluorooctane sulfonate, and 8:2 fluorotelomer alcohol) and four formulations of the PFCs mixtures. Mixtures as well as individual compounds consistently regulated a particular gene set, which suggests that these conserved genes may play a central role in the toxicity mediated by PFCs. Specifically, a number of genes regulated by the mixtures were identified in this study, which were not affected by exposure to any single component. These genes are implicated in multiple biological functions and processes, including fatty acid metabolism and transport, xenobiotic metabolism, immune responses, and oxidative stress. More than 80% of the altered genes in the PFOA- and PFOS-dominant mixture groups were of the same gene set, while the gene expression profiles from single PFOA and PFOS exposures were not as similar. This work contributes to the development of toxicogenomic approaches in combined toxicity assessment and allows for comprehensive insights into the combined action of PFCs mixtures in multiple environmental matrices. (C) 2009 Elsevier B.V. All rights reserved.; Polyfluorinated and perfluorinated compounds (PFCs) are used in numerous commercial products and have been ubiquitously detected in the environment as well as in the blood of humans and wildlife. To assess the combined effects caused by PFCs in mixtures, gene expression profiles were generated using a custom cDNA microarray to detect changes in primary cultured hepatocytes of rare minnows exposed to six individual PFCs (perfluorooctanoic acid, perfluorononanoic acid, perfluorodecanoic acid, perfluorododecanoic acid, perfluorooctane sulfonate, and 8:2 fluorotelomer alcohol) and four formulations of the PFCs mixtures. Mixtures as well as individual compounds consistently regulated a particular gene set, which suggests that these conserved genes may play a central role in the toxicity mediated by PFCs. Specifically, a number of genes regulated by the mixtures were identified in this study, which were not affected by exposure to any single component. These genes are implicated in multiple biological functions and processes, including fatty acid metabolism and transport, xenobiotic metabolism, immune responses, and oxidative stress. More than 80% of the altered genes in the PFOA- and PFOS-dominant mixture groups were of the same gene set, while the gene expression profiles from single PFOA and PFOS exposures were not as similar. This work contributes to the development of toxicogenomic approaches in combined toxicity assessment and allows for comprehensive insights into the combined action of PFCs mixtures in multiple environmental matrices. (C) 2009 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordPolyfluorinated And Perfluorinated Compounds Toxicogenomics Combined Toxicity Primary Cultured Hepatocytes Rare Minnow (Gobiocypris Rarus)
Department[Wei, Yanhong; Zhang, Hongxia; Wang, Jianshe; Dai, Jiayin] Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China; [Shi, Xiongjie; Zhou, Bingsheng] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaMarine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2009.07.020
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [20837004, 20777074] ; National Natural Science Foundation of China [20837004, 20777074] ; National Natural Science Foundation of China [20837004, 20777074] ; National Natural Science Foundation of China [20837004, 20777074]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000271727000004
WOS KeywordPERFLUOROOCTANE SULFONIC-ACID ; GENE-EXPRESSION PROFILES ; SURFACE-WATER SAMPLES ; PEROXISOME PROLIFERATORS ; PERFLUOROALKYL ACIDS ; OXIDATIVE STRESS ; RAINBOW-TROUT ; LIVER ; RAT ; PFOA
Funding OrganizationNational Natural Science Foundation of China [20837004, 20777074] ; National Natural Science Foundation of China [20837004, 20777074] ; National Natural Science Foundation of China [20837004, 20777074] ; National Natural Science Foundation of China [20837004, 20777074]
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7492
Collection期刊论文
Corresponding AuthorDai, JY, Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China
Affiliation1.Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wei, Yanhong,Shi, Xiongjie,Zhang, Hongxia,et al. Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis[J]. AQUATIC TOXICOLOGY,2009,95(1):27-36.
APA Wei, Yanhong.,Shi, Xiongjie.,Zhang, Hongxia.,Wang, Jianshe.,Zhou, Bingsheng.,...&Dai, JY, Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China.(2009).Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis.AQUATIC TOXICOLOGY,95(1),27-36.
MLA Wei, Yanhong,et al."Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis".AQUATIC TOXICOLOGY 95.1(2009):27-36.
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