IHB OpenIR  > 期刊论文
Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish
Wu, Nan; Li, Chang-Jian; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009-12-01
Source PublicationBIOCHEMICAL GENETICS
ISSN0006-2928
Volume47Issue:11-12Pages:749-762
AbstractNucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.; Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.
SubtypeArticle
KeywordOogenesis Spermatogenesis Sperm Decondensation Carassius Auratus Gibelio Carassius Auratus
Department[Wu, Nan; Li, Chang-Jian; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Genetics & Heredity
DOI10.1007/s10528-009-9274-y
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000272015400001
WOS KeywordCARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; CHROMATIN DECONDENSATION ; SPERM DECONDENSATION ; XENOPUS-LAEVIS ; NUCLEOPLASMIN ; PROTEIN ; RNA ; NUCLEOPHOSMIN ; EXPRESSION
Funding OrganizationNational Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7480
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wu, Nan,Li, Chang-Jian,Gui, Jian-Fang,et al. Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish[J]. BIOCHEMICAL GENETICS,2009,47(11-12):749-762.
APA Wu, Nan,Li, Chang-Jian,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish.BIOCHEMICAL GENETICS,47(11-12),749-762.
MLA Wu, Nan,et al."Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish".BIOCHEMICAL GENETICS 47.11-12(2009):749-762.
Files in This Item:
File Name/Size DocType Version Access License
Molecular Characteri(590KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, Nan]'s Articles
[Li, Chang-Jian]'s Articles
[Gui, Jian-Fang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Nan]'s Articles
[Li, Chang-Jian]'s Articles
[Gui, Jian-Fang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Nan]'s Articles
[Li, Chang-Jian]'s Articles
[Gui, Jian-Fang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Molecular Characterization and Functional Commonality of Nucleophosmin-Nucleoplasmin in Two Cyprinid Fish.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.