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学科主题: Biochemistry & Molecular Biology; Genetics & Heredity
题名: Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish
作者: Wu, Nan1; Li, Chang-Jian1; Gui, Jian-Fang1
通讯作者: Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: Oogenesis ; Spermatogenesis ; Sperm decondensation ; Carassius auratus gibelio ; Carassius auratus
刊名: BIOCHEMICAL GENETICS
发表日期: 2009-12-01
DOI: 10.1007/s10528-009-9274-y
卷: 47, 期:11-12, 页:749-762
收录类别: SCI
文章类型: Article
部门归属: [Wu, Nan; Li, Chang-Jian; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]
类目[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
摘要: Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.
英文摘要: Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.
关键词[WOS]: CARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; CHROMATIN DECONDENSATION ; SPERM DECONDENSATION ; XENOPUS-LAEVIS ; NUCLEOPLASMIN ; PROTEIN ; RNA ; NUCLEOPHOSMIN ; EXPRESSION
语种: 英语
WOS记录号: WOS:000272015400001
ISSN号: 0006-2928
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7480
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Wu, Nan; Li, Chang-Jian; Gui, Jian-Fang.Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish,BIOCHEMICAL GENETICS,2009,47(11-12):749-762
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