| Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish | |
| Wu, Nan; Li, Chang-Jian; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China | |
| 2009-12-01 | |
| Source Publication | BIOCHEMICAL GENETICS
 |
| ISSN | 0006-2928 |
| Volume | 47Issue:11-12Pages:749-762 |
| Abstract | Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.; Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish. |
| Subtype | Article |
| Keyword | Oogenesis Spermatogenesis Sperm Decondensation Carassius Auratus Gibelio Carassius Auratus |
| Department | [Wu, Nan; Li, Chang-Jian; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Subject Area | Biochemistry & Molecular Biology ; Genetics & Heredity |
| DOI | 10.1007/s10528-009-9274-y |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS Subject | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS ID | WOS:000272015400001 |
| WOS Keyword | CARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; CHROMATIN DECONDENSATION ; SPERM DECONDENSATION ; XENOPUS-LAEVIS ; NUCLEOPLASMIN ; PROTEIN ; RNA ; NUCLEOPHOSMIN ; EXPRESSION |
| Funding Organization | National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] ; National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/7480 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Nan,Li, Chang-Jian,Gui, Jian-Fang,et al. Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish[J]. BIOCHEMICAL GENETICS,2009,47(11-12):749-762. |
| APA | Wu, Nan,Li, Chang-Jian,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish.BIOCHEMICAL GENETICS,47(11-12),749-762. |
| MLA | Wu, Nan,et al."Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish".BIOCHEMICAL GENETICS 47.11-12(2009):749-762. |
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