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Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp
Peng, Jin-Xia; Xie, Jian-Li; Zhou, Li; Hong, Yun-Han; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China
2009
Source PublicationJOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
Volume312BIssue:8Pages:855-871
AbstractTo investigate germline development and germ cell specification, we identified a Dazl homolog (CagDazl) from gynogenetic gibel carp (Carassius auratus gibelio). Its cDNA sequence and BAC clone sequence analyses revealed the genomic organization conservation and conserved synteny of the Dazl family members and their neighborhood genes among vertebrates, especially in fish. Moreover, a polyclonal antibody specific to CagDazl was produced and used to examine its expression and distribution throughout germline development at protein level. Firstly, ovary-specific expression pattern of CagDazl was confirmed in adult tissues by RT-PCR and Western blot. In addition, in situ hybridization and immunofluorescence localization demonstrated its specific expression in germ cells, and both its transcript and protein were localized to germ plasm. Then, co-localization of CagDazl and mitochondrial cloud was found, confirming that CagDazl transcript and its protein are germ plasm component and move via METRO pathway during oogenesis. Furthermore, the CagDazl is abundant and continuous throughout germline development and germ cell specification including primordial germ cell (PGC) formation, oogonium differentiation, oocyte development, and embryogenesis, and the dynamic distribution occurs at different development stages. The data suggest that maternal CagDazl might play an important role in gibel carp PGC formation. Therefore, CagDazl is a useful and specific marker for tracing germ plasm and germ cell development in the gynogenetic gibel carp. In addition, in comparison with previous studies in sexual reproduction species, the continuous and dynamic distribution of CagDazl protein in the germ plasm throughout the life cycle seems to have significant implication in sex evolution of vertebrates. J. Exp. Zool. (Mol. Deu. Euol.) 312B:855-871, 2009. (C) 2009 Wiley-Liss, Inc.
KeywordCarassius-auratus-gibelio Silver Crucian Carp Xenopus Oocytes Mitochondrial Cloud Rna Localization Cell Development Binding Protein Family Proteins Vegetal Cortex Gene Encodes
Department[Peng, Jin-Xia; Xie, Jian-Li; Zhou, Li; Hong, Yun-Han; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Hubei, Peoples R China; [Hong, Yun-Han] Natl Univ Singapore, Dept Biol Sci, Singapore 0511, Singapore
Subject AreaEvolutionary Biology ; Developmental Biology ; Zoology
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology
Indexed BySCI
Language英语
WOS IDWOS:000271958800006
Funding OrganizationNational Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology ; National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301, 085A021301]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ15]; Institute of Hydrobiology
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Cited Times:50[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7474
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Peng, Jin-Xia,Xie, Jian-Li,Zhou, Li,et al. Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,2009,312B(8):855-871.
APA Peng, Jin-Xia,Xie, Jian-Li,Zhou, Li,Hong, Yun-Han,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China.(2009).Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp.JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,312B(8),855-871.
MLA Peng, Jin-Xia,et al."Evolutionary Conservation of Dazl Genomic Organization and its Continuous and Dynamic Distribution Throughout Germline Development in Gynogenetic Gibel Carp".JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION 312B.8(2009):855-871.
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