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学科主题: Immunology; Veterinary Sciences
题名: Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation
作者: Tian, J. Y.1, 2, 3; Xie, H. X.1, 2; Zhang, Y. A.1, 2; Xu, Z.1, 2; Yao, W. J.1, 2; Nie, P.1, 2
通讯作者: Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
关键词: IgM-producing cell ; Ontogeny ; In situ hybridisation ; Mandarin fish ; Siniperca chuatsi
刊名: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
发表日期: 2009-12-15
DOI: 10.1016/j.vetimm.2009.05.018
卷: 132, 期:2-4, 页:146-152
收录类别: SCI
文章类型: Article
部门归属: [Tian, J. Y.; Xie, H. X.; Zhang, Y. A.; Xu, Z.; Yao, W. J.; Nie, P.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Tian, J. Y.; Xie, H. X.; Zhang, Y. A.; Xu, Z.; Yao, W. J.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Immunol & Parasitol, Wuhan 430072, Hubei Province, Peoples R China; [Tian, J. Y.] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province
类目[WOS]: Immunology ; Veterinary Sciences
研究领域[WOS]: Immunology ; Veterinary Sciences
摘要: The ontogeny of IgM-producing cells was studied in juvenile mandarin fish Simperca chuatsi, an important fish in China's aquaculture sector. The IgM-producing cells were localised through in situ hybridisation with a probe complementary to the Ig mu-chain in lymphoid-related tissues, including head kidney, spleen, thymus, intestine and gills. In head kidney, transcripts of Ig mu were first detected at 20 days post-hatching (dph) with a few positive signals. and the number of IgM-producing cells increased obviously from 39 dph onwards. At 136 dph, a large amount of positive cells were observed in the entire organ with clusters of these cells located around the blood vessels. In spleen, IgM-producing cells were found from 26 dph onwards, followed by an increase until 67 dph: clusters of positive cells were also detected around blood vessels at 102 dph. In thymus, IgM-producing cells were first observed at 39 dph; thereafter, no obvious increase was detected until 78 dph. The positive cells in thymus were distributed mainly in the outer zone of thymus. A few IgM-producing cells were still observed in thymus of 1-year-old mandarin fish. IgM-producing cells were not detected in the intestine until 87 dph, with several discrete positively stained cells distributed in the lamina propria. IgM-producing cells, scattered mainly in primary gill filaments around blood vessels, were detected in gills from 90 dph. As in other teleosts, these results indicated that the head kidney appears to be the primary organ for IgM production in mandarin fish, and IgM-producing cells exist in all organs examined in the present study, implying their lymphoid role in fish. In addition, it is suggested that vaccination after 20 dph may be much more effective in mandarin fish. (C) 2009 Elsevier B.V. All rights reserved.
英文摘要: The ontogeny of IgM-producing cells was studied in juvenile mandarin fish Simperca chuatsi, an important fish in China's aquaculture sector. The IgM-producing cells were localised through in situ hybridisation with a probe complementary to the Ig mu-chain in lymphoid-related tissues, including head kidney, spleen, thymus, intestine and gills. In head kidney, transcripts of Ig mu were first detected at 20 days post-hatching (dph) with a few positive signals. and the number of IgM-producing cells increased obviously from 39 dph onwards. At 136 dph, a large amount of positive cells were observed in the entire organ with clusters of these cells located around the blood vessels. In spleen, IgM-producing cells were found from 26 dph onwards, followed by an increase until 67 dph: clusters of positive cells were also detected around blood vessels at 102 dph. In thymus, IgM-producing cells were first observed at 39 dph; thereafter, no obvious increase was detected until 78 dph. The positive cells in thymus were distributed mainly in the outer zone of thymus. A few IgM-producing cells were still observed in thymus of 1-year-old mandarin fish. IgM-producing cells were not detected in the intestine until 87 dph, with several discrete positively stained cells distributed in the lamina propria. IgM-producing cells, scattered mainly in primary gill filaments around blood vessels, were detected in gills from 90 dph. As in other teleosts, these results indicated that the head kidney appears to be the primary organ for IgM production in mandarin fish, and IgM-producing cells exist in all organs examined in the present study, implying their lymphoid role in fish. In addition, it is suggested that vaccination after 20 dph may be much more effective in mandarin fish. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: IMMUNOGLOBULIN LIGHT-CHAIN ; ANARHICHAS-MINOR OLAFSEN ; GADUS-MORHUA L ; RAINBOW-TROUT ; LYMPHOID ORGANS ; CHANNEL CATFISH ; POSITIVE CELLS ; GENE STRUCTURE ; BEARING CELLS ; HEAVY-CHAIN
语种: 英语
WOS记录号: WOS:000272861100009
ISSN号: 0165-2427
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/7448
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Immunol & Parasitol, Wuhan 430072, Hubei Province, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

Recommended Citation:
Tian, J. Y.; Xie, H. X.; Zhang, Y. A.; Xu, Z.; Yao, W. J.; Nie, P..Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation,VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2009,132(2-4):146-152
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