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Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation
Tian, J. Y.1,2,3; Xie, H. X.1,2; Zhang, Y. A.1,2; Xu, Z.1,2; Yao, W. J.1,2; Nie, P.1,2; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2009-12-15
Source PublicationVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN0165-2427
Volume132Issue:2-4Pages:146-152
AbstractThe ontogeny of IgM-producing cells was studied in juvenile mandarin fish Simperca chuatsi, an important fish in China's aquaculture sector. The IgM-producing cells were localised through in situ hybridisation with a probe complementary to the Ig mu-chain in lymphoid-related tissues, including head kidney, spleen, thymus, intestine and gills. In head kidney, transcripts of Ig mu were first detected at 20 days post-hatching (dph) with a few positive signals. and the number of IgM-producing cells increased obviously from 39 dph onwards. At 136 dph, a large amount of positive cells were observed in the entire organ with clusters of these cells located around the blood vessels. In spleen, IgM-producing cells were found from 26 dph onwards, followed by an increase until 67 dph: clusters of positive cells were also detected around blood vessels at 102 dph. In thymus, IgM-producing cells were first observed at 39 dph; thereafter, no obvious increase was detected until 78 dph. The positive cells in thymus were distributed mainly in the outer zone of thymus. A few IgM-producing cells were still observed in thymus of 1-year-old mandarin fish. IgM-producing cells were not detected in the intestine until 87 dph, with several discrete positively stained cells distributed in the lamina propria. IgM-producing cells, scattered mainly in primary gill filaments around blood vessels, were detected in gills from 90 dph. As in other teleosts, these results indicated that the head kidney appears to be the primary organ for IgM production in mandarin fish, and IgM-producing cells exist in all organs examined in the present study, implying their lymphoid role in fish. In addition, it is suggested that vaccination after 20 dph may be much more effective in mandarin fish. (C) 2009 Elsevier B.V. All rights reserved.; The ontogeny of IgM-producing cells was studied in juvenile mandarin fish Simperca chuatsi, an important fish in China's aquaculture sector. The IgM-producing cells were localised through in situ hybridisation with a probe complementary to the Ig mu-chain in lymphoid-related tissues, including head kidney, spleen, thymus, intestine and gills. In head kidney, transcripts of Ig mu were first detected at 20 days post-hatching (dph) with a few positive signals. and the number of IgM-producing cells increased obviously from 39 dph onwards. At 136 dph, a large amount of positive cells were observed in the entire organ with clusters of these cells located around the blood vessels. In spleen, IgM-producing cells were found from 26 dph onwards, followed by an increase until 67 dph: clusters of positive cells were also detected around blood vessels at 102 dph. In thymus, IgM-producing cells were first observed at 39 dph; thereafter, no obvious increase was detected until 78 dph. The positive cells in thymus were distributed mainly in the outer zone of thymus. A few IgM-producing cells were still observed in thymus of 1-year-old mandarin fish. IgM-producing cells were not detected in the intestine until 87 dph, with several discrete positively stained cells distributed in the lamina propria. IgM-producing cells, scattered mainly in primary gill filaments around blood vessels, were detected in gills from 90 dph. As in other teleosts, these results indicated that the head kidney appears to be the primary organ for IgM production in mandarin fish, and IgM-producing cells exist in all organs examined in the present study, implying their lymphoid role in fish. In addition, it is suggested that vaccination after 20 dph may be much more effective in mandarin fish. (C) 2009 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordIgm-producing Cell Ontogeny In Situ Hybridisation Mandarin Fish Siniperca Chuatsi
Department[Tian, J. Y.; Xie, H. X.; Zhang, Y. A.; Xu, Z.; Yao, W. J.; Nie, P.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Tian, J. Y.; Xie, H. X.; Zhang, Y. A.; Xu, Z.; Yao, W. J.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Immunol & Parasitol, Wuhan 430072, Hubei Province, Peoples R China; [Tian, J. Y.] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China
Subject AreaImmunology ; Veterinary Sciences
DOI10.1016/j.vetimm.2009.05.018
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Veterinary Sciences
WOS SubjectImmunology ; Veterinary Sciences
WOS IDWOS:000272861100009
WOS KeywordIMMUNOGLOBULIN LIGHT-CHAIN ; ANARHICHAS-MINOR OLAFSEN ; GADUS-MORHUA L ; RAINBOW-TROUT ; LYMPHOID ORGANS ; CHANNEL CATFISH ; POSITIVE CELLS ; GENE STRUCTURE ; BEARING CELLS ; HEAVY-CHAIN
Funding OrganizationNational Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province
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Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/7448
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Immunol & Parasitol, Wuhan 430072, Hubei Province, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Tian, J. Y.,Xie, H. X.,Zhang, Y. A.,et al. Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2009,132(2-4):146-152.
APA Tian, J. Y..,Xie, H. X..,Zhang, Y. A..,Xu, Z..,Yao, W. J..,...&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.(2009).Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,132(2-4),146-152.
MLA Tian, J. Y.,et al."Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 132.2-4(2009):146-152.
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