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豌豆核酮糖1,5-二磷酸羧化酶/加氧酶亚基结合蛋白的纯化及特性
赵若虹,缪有刚,李立人
1991
Source Publication植物生理与分子生物学学报
Issue3
Abstract用~(35)S-Met在照光下与豌豆完整叶绿体保温,显示新合成的标记的RubisCO大亚基与结合蛋白形成一复合物,经ATP处理后解离为结合蛋白亚基,同时释放出的标记的RubisCO大亚基参与了RubisCO的装配。豌豆叶片提取液经热处理,硫酸铵分部,DEAE-Sepharose fast flow和Sephacryl S-300柱层析在ND-PAGE,SDS-PAGE上显示为一条带,估计纯度达90%以上,得率比以前报道的高12倍。纯化的结合蛋白表面巯基数经测定为12±1个,总巯基数为36±1个。远紫外CD
Keyword核酮糖1 5-二磷酸羧化酶/加氧酶亚基结合蛋白 纯化 装配 特性
Department中国科学院上海植物生理研究所;中国科学院水生生物研究所
Indexed ByCSCD
Language中文
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/5690
Collection期刊论文
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GB/T 7714
赵若虹,缪有刚,李立人. 豌豆核酮糖1,5-二磷酸羧化酶/加氧酶亚基结合蛋白的纯化及特性[J]. 植物生理与分子生物学学报,1991(3).
APA 赵若虹,缪有刚,李立人.(1991).豌豆核酮糖1,5-二磷酸羧化酶/加氧酶亚基结合蛋白的纯化及特性.植物生理与分子生物学学报(3).
MLA 赵若虹,缪有刚,李立人."豌豆核酮糖1,5-二磷酸羧化酶/加氧酶亚基结合蛋白的纯化及特性".植物生理与分子生物学学报 .3(1991).
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