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总DNA介导鱼类基因转移的初步研究
刘汉勤; 郭文; 王铁辉; 陈宏溪
1991
Source Publication水生生物学报
Issue3
Abstract<正> 自七十年代我国开始将外源总DNA转移技术应用于棉花和水稻品种改良的研究中,但至今尚未见有关鱼类总DNA转移成功的报道。本实验采用显微注射和精于载体的方法将鲫鱼肝总DNA转移到红鲤受精卵,利用鯽鱼和红鲤在孵化前后色素表达的差异来筛选转移基因个体。此方法无需克隆目的基因及DNA重组操作,而且筛选简便,目的在于探讨总DNA转移方法应用于鱼类品种改良的可能性。
Keyword总dna 鱼类 显微注射 精子载体 基因转移
Department中国科学院水生生物研究所
Indexed ByCSCD
Language中文
CSCD IDCSCD:155754
Citation statistics
Cited Times:6[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/5620
Collection期刊论文
Recommended Citation
GB/T 7714
刘汉勤,郭文,王铁辉,等. 总DNA介导鱼类基因转移的初步研究[J]. 水生生物学报,1991(3).
APA 刘汉勤,郭文,王铁辉,&陈宏溪.(1991).总DNA介导鱼类基因转移的初步研究.水生生物学报(3).
MLA 刘汉勤,et al."总DNA介导鱼类基因转移的初步研究".水生生物学报 .3(1991).
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