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学科主题: Environmental Sciences; Toxicology
题名: CYCLIC PEPTIDE HEPATOTOXINS FROM FRESH-WATER CYANOBACTERIAL (BLUE-GREEN-ALGAE) WATERBLOOMS COLLECTED IN CENTRAL CHINA
作者: QING-XUE ZHANG ; WAYNE W.CARMICHAEL ; MIN-JUAN YU ; HANG-HAO LI
关键词: CYANOBACTERIA ; BLUE-GREEN ALGAE ; WATERBLOOMS ; HEPATOTOXINS
刊名: ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
发表日期: 1991
卷: 10, 期:3, 页:313-321
收录类别: SCI
部门归属: WRIGHT STATE UNIV,DEPT BIOL SCI,DAYTON,OH 45435; ACAD SINICA,INST HYDROBIOL,WUHAN,PEOPLES R CHINA
摘要: Toxic cyanobacteria (blue-green algae) waterblooms have been found in several Chinese water bodies since studies began there in 1984. Waterbloom samples for this study contained Anabaena circinalis, Microcystis aeruginosa and Oscillatoria sp. Only those waterblooms dominated by Microcystis aeruginosa were toxic by the intraperitoneal (i.p.) mouse bioassay. Signs of poisoning were the same as with known hepatotoxic cyclic peptide microcystins. One toxic fraction was isolated from each Microcystis aeruginosa sample. Two hepatotoxic peptides were purified from each of the fractions by high-performance liquid chromatography and identified by amino acid analysis followed by low and high resolution fast-atom bombardment mass spectrometry (FAB-MS). LD50 i.p. mouse values for the two toxins were 245-mu-g/kg (Toxin A) and 53-mu-g/g (Toxin B). Toxin content in the cells was 0.03 to 3.95 mg/g (Toxin A) and 0.18 to 3.33 mg/kg (Toxin B). The amino acid composition of Toxin A was alanine [1], arginine [2], glutamic acid [1] and beta-methylaspartic acid [1]; for Toxin B it was the same, except one of the arginines was replaced with a leucine. Low- and high-resolution FAB-MS showed that the molecular weights were 1,037 m/z (Toxin A) and 994 m/z (Toxin B), with formulas of C49H76O12N13 (Toxin A) and C49H75O12N10 (Toxin B). It was concluded that Toxin A is microcystin-RR and Toxin B is microcystin-LR, both known cyclic heptapeptide hepatotoxins isolated from cyanobacteria in other parts of the world. Sodium borohydride reduction of microcystin-RR yielded dihydro-microcystin-RR (m/z = 1,039), an important intermediate in the preparation of tritium-labeled toxin for metabolism and fate studies.
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/10360
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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QING-XUE ZHANG; WAYNE W.CARMICHAEL; MIN-JUAN YU; HANG-HAO LI.CYCLIC PEPTIDE HEPATOTOXINS FROM FRESH-WATER CYANOBACTERIAL (BLUE-GREEN-ALGAE) WATERBLOOMS COLLECTED IN CENTRAL CHINA,ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,1991,10(3):313-321
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文件名: CYCLIC PEPTIDE HEPATOTOXINS FROM FRESH-WATER CYANOBACTERIAL (BLUE-GREEN-ALGAE) WATERBLOOMS COLLECTED IN CENTRAL CHINA.pdf
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