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CYCLIC PEPTIDE HEPATOTOXINS FROM FRESH-WATER CYANOBACTERIAL (BLUE-GREEN-ALGAE) WATERBLOOMS COLLECTED IN CENTRAL CHINA
QING-XUE ZHANG; WAYNE W.CARMICHAEL; MIN-JUAN YU; HANG-HAO LI
1991
Source PublicationENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
Volume10Issue:3Pages:313-321
AbstractToxic cyanobacteria (blue-green algae) waterblooms have been found in several Chinese water bodies since studies began there in 1984. Waterbloom samples for this study contained Anabaena circinalis, Microcystis aeruginosa and Oscillatoria sp. Only those waterblooms dominated by Microcystis aeruginosa were toxic by the intraperitoneal (i.p.) mouse bioassay. Signs of poisoning were the same as with known hepatotoxic cyclic peptide microcystins. One toxic fraction was isolated from each Microcystis aeruginosa sample. Two hepatotoxic peptides were purified from each of the fractions by high-performance liquid chromatography and identified by amino acid analysis followed by low and high resolution fast-atom bombardment mass spectrometry (FAB-MS). LD50 i.p. mouse values for the two toxins were 245-mu-g/kg (Toxin A) and 53-mu-g/g (Toxin B). Toxin content in the cells was 0.03 to 3.95 mg/g (Toxin A) and 0.18 to 3.33 mg/kg (Toxin B). The amino acid composition of Toxin A was alanine [1], arginine [2], glutamic acid [1] and beta-methylaspartic acid [1]; for Toxin B it was the same, except one of the arginines was replaced with a leucine. Low- and high-resolution FAB-MS showed that the molecular weights were 1,037 m/z (Toxin A) and 994 m/z (Toxin B), with formulas of C49H76O12N13 (Toxin A) and C49H75O12N10 (Toxin B). It was concluded that Toxin A is microcystin-RR and Toxin B is microcystin-LR, both known cyclic heptapeptide hepatotoxins isolated from cyanobacteria in other parts of the world. Sodium borohydride reduction of microcystin-RR yielded dihydro-microcystin-RR (m/z = 1,039), an important intermediate in the preparation of tritium-labeled toxin for metabolism and fate studies.
KeywordCyanobacteria Blue-green Algae Waterblooms Hepatotoxins
DepartmentWRIGHT STATE UNIV,DEPT BIOL SCI,DAYTON,OH 45435; ACAD SINICA,INST HYDROBIOL,WUHAN,PEOPLES R CHINA
Subject AreaEnvironmental Sciences ; Toxicology
Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10360
Collection期刊论文
Recommended Citation
GB/T 7714
QING-XUE ZHANG,WAYNE W.CARMICHAEL,MIN-JUAN YU,et al. CYCLIC PEPTIDE HEPATOTOXINS FROM FRESH-WATER CYANOBACTERIAL (BLUE-GREEN-ALGAE) WATERBLOOMS COLLECTED IN CENTRAL CHINA[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,1991,10(3):313-321.
APA QING-XUE ZHANG,WAYNE W.CARMICHAEL,MIN-JUAN YU,&HANG-HAO LI.(1991).CYCLIC PEPTIDE HEPATOTOXINS FROM FRESH-WATER CYANOBACTERIAL (BLUE-GREEN-ALGAE) WATERBLOOMS COLLECTED IN CENTRAL CHINA.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,10(3),313-321.
MLA QING-XUE ZHANG,et al."CYCLIC PEPTIDE HEPATOTOXINS FROM FRESH-WATER CYANOBACTERIAL (BLUE-GREEN-ALGAE) WATERBLOOMS COLLECTED IN CENTRAL CHINA".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 10.3(1991):313-321.
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