Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; GSF Forschungszentrum Umwelt & Gesundheit, Inst Ecol Chem, D-85764 Neuherberg, Germany
Several biochemical responses were measured in silver crucian carp (Carassius auratus gibelio) after exposure to sediments obtained from contaminated Ya-Er Lake, No, 1 pond, and an unpolluted reference site, Honglian Lake. After 1 week of exposure, a significant induction of the phase I biotransformation enzyme (ethoxylresorufin-o-deethylase, EROD) was found (83-fold of control), whereas the phase II biotransformation enzyme (glutathione S-transferase, GST) exhibited a slight, but significant induction (1,4-fold of control) after 4 weeks of exposure. The level of cellular glutathione in the liver was also slightly elevated after 4 weeks of exposure. The delayed response of GST to the contaminants indicates that the phase I and phase II biotransformation enzymes are regulated differently in fish. The results suggest that EROD is a sensitive bioindicator to assess the toxicity of dioxin-contamined sediment in the laboratory, (C) 1998 Academic Press.
Chen Guosheng; Xu Ying; Xu Lihong; Zhang Yongyuan; Karl-Werner Schramm and Antonius Kettrup.Influence of dioxin and metal-contaminated sediment on phase I and II biotransformation enzymes in silver crucian carp,ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,1998,40(3):234-238