| Studies on polysaccharides from three edible species of Nostoc (Cyanobacteria) with different colony morphologies: Comparison of monosaccharide compositions and viscosities of polysaccharides from field colonies and suspension cultures | |
| Zebo Huang; Yongding Liu; Berit Smestad Paulsen; Dag Klaveness; Paulsen, BS, Univ Oslo, Inst Pharm, PO 1068, N-0316 Oslo, Norway | |
| 1998 | |
| Source Publication | JOURNAL OF PHYCOLOGY
 |
| Volume | 34Issue:6Pages:962-968 |
| Abstract | Hot water-soluble polysaccharides woe extracted from field colonies and suspension cultures of Nostoc commune Vaucher, Nostoc flagelliforme Berkeley et Curtis, and Nostoc sphaeroides Kutzing. Excreted extracellular polymeric substances (EPS) were isolated from the media in which the suspension cultures were grown. The main monosaccharides of the field colony polysaccharides from the three species were glucose, xylose, and galactose, with an approximate ratio of 2:1:1. Mannose was also present, but the levels varied among the species, and arabinose appeared only in N. flagelliforme. The compositions of the cellular polysaccharides and EPS from suspension cultures were more complicated than those of the field samples and varied among the different species. The polysaccharides from the cultures of N. flagelliforme had a relatively simple composition consisting of mannose, galactose, glucose, and glucuronic acid, but no xylose, as was found in the field colony polysaccharides. The polysaccharides from cultures of N. sphaeroides contained glucose (the major component), rhamnose, fucose, xylose, mannose, and galactose. These same sugars were present in the polysaccharides from cultures of N. commune, with xylose as the major component. Combined nitrogen in the media had no qualitative influence on the compositions of the cellular polysaccharides but affected those of the EPS of N. commune and N. flagelliforme. The EPS of N. sphaeroides had a very low fetal carbohydrate content and thus was not considered to be polysaccharide in nature. The field colony polysaccharides could be separated by anion exchange chromatography into neutral and acidic fractions having similar sugar compositions. Preliminary linkage analysis showed that 1) xylose, glucose, and galactose were 1-->4 linked, 2) mannose, galactose, and xylose occurred as terminal residues, and 3) branch points occurred in glucose as 1-->3,4 and 1-->3,6 linkages and in xylose as a 1-->3,4 linkage. The polymer preparations from field colonies had higher kinematic viscosities than those from corresponding suspension cultures. The high viscosities of the polymers suggested that they might DE suitable for industrial uses. |
| Keyword | Cyanobacteria Extracellular Polymers Field Colony Monosaccharide Composition Nostoc Polysaccharides Suspension Culture Viscosity |
| Department | Univ Oslo, Inst Pharm, N-0316 Oslo, Norway; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Univ Oslo, Dept Biol, N-0315 Oslo, Norway |
| Subject Area | Plant Sciences ; Marine & Freshwater Biology |
| Indexed By | SCI |
| WOS ID | WOS:000077966100008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/10208 |
| Collection | 期刊论文 |
| Corresponding Author | Paulsen, BS, Univ Oslo, Inst Pharm, PO 1068, N-0316 Oslo, Norway |
| Recommended Citation GB/T 7714 | Zebo Huang,Yongding Liu,Berit Smestad Paulsen,et al. Studies on polysaccharides from three edible species of Nostoc (Cyanobacteria) with different colony morphologies: Comparison of monosaccharide compositions and viscosities of polysaccharides from field colonies and suspension cultures[J]. JOURNAL OF PHYCOLOGY,1998,34(6):962-968. |
| APA | Zebo Huang,Yongding Liu,Berit Smestad Paulsen,Dag Klaveness,&Paulsen, BS, Univ Oslo, Inst Pharm, PO 1068, N-0316 Oslo, Norway.(1998).Studies on polysaccharides from three edible species of Nostoc (Cyanobacteria) with different colony morphologies: Comparison of monosaccharide compositions and viscosities of polysaccharides from field colonies and suspension cultures.JOURNAL OF PHYCOLOGY,34(6),962-968. |
| MLA | Zebo Huang,et al."Studies on polysaccharides from three edible species of Nostoc (Cyanobacteria) with different colony morphologies: Comparison of monosaccharide compositions and viscosities of polysaccharides from field colonies and suspension cultures".JOURNAL OF PHYCOLOGY 34.6(1998):962-968. |
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