| Comparative study on in vitro inhibition of grass carp (Ctenopharyngodon idellus) and mouse protein phosphatases by microcystins | |
| L. Xu; P. K. S. Lam; J. Chen; Y. Zhang; K. Harada; Lam, PKS, Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China | |
| 2000 | |
| Source Publication | ENVIRONMENTAL TOXICOLOGY
 |
| Volume | 15Issue:2Pages:71-75 |
| Abstract | Microcystins isolated from toxic cyanobacteria are potent inhibitors of protein phosphatases 1 and 2A (PP1 and PP2A). The inhibitory effects of three structural variants of microcystins (microcystin-LR, -YR, and -RR) on protein phosphatases isolated and purified from the liver and kidney of grass carp (Ctenopharyngodon idellus) were investigated using the P-32 radiometric assay. The relationships between percentage inhibition of protein phosphatase activity and microcystin levels followed a typical dose-dependent sigmoid curve. These results were compared to those obtained from mouse PP2A. The degree and pattern of inhibition of both fish and mouse protein phosphatases by microcystins were similar. Protein phosphatases in crude fish tissue homogenates showed similar inhibition patterns as purified fish PP2A toward microcystins. (C) 2000 by John Wiley & Sons, Inc. |
| Keyword | Microcystins Fish Protein Phosphatases In Vitro Inhibition |
| Department | Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; City Univ Hong Kong, Ctr Environm Sci & Technol, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China; Meijo Univ, Fac Pharm, Nagoya, Aichi 468, Japan |
| Subject Area | Environmental Sciences ; Toxicology ; Water Resources |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000086543900001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/10136 |
| Collection | 期刊论文 |
| Corresponding Author | Lam, PKS, Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| Recommended Citation GB/T 7714 | L. Xu,P. K. S. Lam,J. Chen,et al. Comparative study on in vitro inhibition of grass carp (Ctenopharyngodon idellus) and mouse protein phosphatases by microcystins[J]. ENVIRONMENTAL TOXICOLOGY,2000,15(2):71-75. |
| APA | L. Xu,P. K. S. Lam,J. Chen,Y. Zhang,K. Harada,&Lam, PKS, Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.(2000).Comparative study on in vitro inhibition of grass carp (Ctenopharyngodon idellus) and mouse protein phosphatases by microcystins.ENVIRONMENTAL TOXICOLOGY,15(2),71-75. |
| MLA | L. Xu,et al."Comparative study on in vitro inhibition of grass carp (Ctenopharyngodon idellus) and mouse protein phosphatases by microcystins".ENVIRONMENTAL TOXICOLOGY 15.2(2000):71-75. |
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