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Time course of foreign gene integration and expression in transgenic fish embryos
ZHAO Haobin; CHEN Shangping; SUN Yonghua; WANG Yaping & ZHU Zuoyan; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2000
Source PublicationCHINESE SCIENCE BULLETIN
Volume45Issue:8Pages:734-739
AbstractUsing a nuclear transplantation approach, the integration and expression of the green fluorescent protein (GFP) gene in the embryogenesis of transgenic leach (Misgurnus anguillicaudatus Cantor) have been studied. The GFP gene expression is first observed at the gastrula stage, which is consistent with the initiation of cell differentiation of fish embryos. The time course of the foreign gene expression is correlated with the regulatory sequences. The expression efficiency also depends on the gene configuration: the expression of pre-integrating circular plasmid at early embryos is higher than that of the linear plasmid. The integration of the GFP gene is first detected at the blastula stage and lasts for quite a long period. When two types of different plasmids are co-injected into fertilized eggs, the behavior of their integration and expression is not identical.
KeywordGene Integration And Expression Time Course Nuclear Transplantation Fish Gfp
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
Indexed BySCI
WOS IDWOS:000087521700011
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10122
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
ZHAO Haobin,CHEN Shangping,SUN Yonghua,et al. Time course of foreign gene integration and expression in transgenic fish embryos[J]. CHINESE SCIENCE BULLETIN,2000,45(8):734-739.
APA ZHAO Haobin,CHEN Shangping,SUN Yonghua,WANG Yaping & ZHU Zuoyan,&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2000).Time course of foreign gene integration and expression in transgenic fish embryos.CHINESE SCIENCE BULLETIN,45(8),734-739.
MLA ZHAO Haobin,et al."Time course of foreign gene integration and expression in transgenic fish embryos".CHINESE SCIENCE BULLETIN 45.8(2000):734-739.
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