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学科主题: Multidisciplinary Sciences
题名: Time course of foreign gene integration and expression in transgenic fish embryos
作者: ZHAO Haobin ; CHEN Shangping ; SUN Yonghua ; WANG Yaping & ZHU Zuoyan
通讯作者: Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: gene integration and expression ; time course ; nuclear transplantation ; fish ; GFP
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2000
卷: 45, 期:8, 页:734-739
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
摘要: Using a nuclear transplantation approach, the integration and expression of the green fluorescent protein (GFP) gene in the embryogenesis of transgenic leach (Misgurnus anguillicaudatus Cantor) have been studied. The GFP gene expression is first observed at the gastrula stage, which is consistent with the initiation of cell differentiation of fish embryos. The time course of the foreign gene expression is correlated with the regulatory sequences. The expression efficiency also depends on the gene configuration: the expression of pre-integrating circular plasmid at early embryos is higher than that of the linear plasmid. The integration of the GFP gene is first detected at the blastula stage and lasts for quite a long period. When two types of different plasmids are co-injected into fertilized eggs, the behavior of their integration and expression is not identical.
WOS记录号: WOS:000087521700011
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/10122
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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ZHAO Haobin; CHEN Shangping; SUN Yonghua; WANG Yaping & ZHU Zuoyan.Time course of foreign gene integration and expression in transgenic fish embryos,CHINESE SCIENCE BULLETIN,2000,45(8):734-739
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