Univ Mediterranee, Fac Med Marseille, Lab Virol Mol Trop & Transfus, F-13005 Marseille 5, France; EFS Alpes Mediterranee, Unite Virus Emergents, Mol Virol Lab, F-13005 Marseille, France; CAS, Wuhan Inst Virol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China; CAS, Wuhan Inst Virol, Inst Hydrobiol, Wuhan 430071, Peoples R China
The genome segments 1, 2, and 3 of the grass carp reovirus (GCRV), a tentative species assigned to genus Aquareouirus, family Reouiridae, were sequenced. The respective segments 1, 2, and 3 were 3949, 3877, and 3702 nucleotides long. Conserved moths 5' (GUUAUUU) and 3' (UUCAUC) were found at the ends of each segment. Each segment contains a single ORF and the negative strand does not permit identification of consistent ORFs. Sequence analysis revealed that VP2 is the viral polymerase, while VPI might represent the viral guanyly/methyl transferase (involved in the capping process of RNA transcripts) and VP3 the NTPase/helicase (involved in the transcription and capping of viral RNAs), The highest amino acid identities (26-41%) were found with orthoreovirus proteins. Further genomic characterization should provide insight about the genetic relationships between GCRV, aquareoviruses, and orthoreoviruses, It should also permit to precise the taxonomic status of these different viruses. (C) 2000 Academic Press.