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Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control
Xu, XD; Yan, G; Kong, RQ; Liu, XP; Yu, LH; Xu, XD, Shandong Inst Parasit Dis, Shandong 272133, Peoples R China
2000
Source PublicationCURRENT MICROBIOLOGY
Volume41Issue:5Pages:352-356
AbstractAnabaena strains expressing the binary toxin genes of Bacillus sphaericus produce high larvicidal activity with living cells. Western blot analysis showed that the 51-kDa and 42-kDa toxin proteins were stable in Anabaena. When a DNA fragment upstream of the 51-kDa protein gene was deleted, the toxicity was reduced by over a hundred-fold, whereas deletions at the coding regions showed that the cooperation of the two proteins expressed in Anabaena is essential for the larvicidal activity. Outdoor tests showed that the genetically altered Anabaena could keep containers with natural water from being inhabited by Culex larvae for over 2 months.
KeywordThuringiensis Subsp Israelensis
DepartmentShandong Inst Parasit Dis, Shandong 272133, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Subject AreaMicrobiology
Indexed BySCI
WOS IDWOS:000089989600009
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10078
Collection期刊论文
Corresponding AuthorXu, XD, Shandong Inst Parasit Dis, Shandong 272133, Peoples R China
Recommended Citation
GB/T 7714
Xu, XD,Yan, G,Kong, RQ,et al. Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control[J]. CURRENT MICROBIOLOGY,2000,41(5):352-356.
APA Xu, XD,Yan, G,Kong, RQ,Liu, XP,Yu, LH,&Xu, XD, Shandong Inst Parasit Dis, Shandong 272133, Peoples R China.(2000).Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control.CURRENT MICROBIOLOGY,41(5),352-356.
MLA Xu, XD,et al."Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control".CURRENT MICROBIOLOGY 41.5(2000):352-356.
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