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学科主题: Microbiology
题名: Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control
作者: Xu, XD; Yan, G; Kong, RQ; Liu, XP; Yu, LH
通讯作者: Xu, XD, Shandong Inst Parasit Dis, Shandong 272133, Peoples R China
关键词: THURINGIENSIS SUBSP ISRAELENSIS
刊名: CURRENT MICROBIOLOGY
发表日期: 2000-11-01
卷: 41, 期:5, 页:352-356
收录类别: SCI
文章类型: Article
部门归属: Shandong Inst Parasit Dis, Shandong 272133, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: Anabaena strains expressing the binary toxin genes of Bacillus sphaericus produce high larvicidal activity with living cells. Western blot analysis showed that the 51-kDa and 42-kDa toxin proteins were stable in Anabaena. When a DNA fragment upstream of the 51-kDa protein gene was deleted, the toxicity was reduced by over a hundred-fold, whereas deletions at the coding regions showed that the cooperation of the two proteins expressed in Anabaena is essential for the larvicidal activity. Outdoor tests showed that the genetically altered Anabaena could keep containers with natural water from being inhabited by Culex larvae for over 2 months.
英文摘要: Anabaena strains expressing the binary toxin genes of Bacillus sphaericus produce high larvicidal activity with living cells. Western blot analysis showed that the 51-kDa and 42-kDa toxin proteins were stable in Anabaena. When a DNA fragment upstream of the 51-kDa protein gene was deleted, the toxicity was reduced by over a hundred-fold, whereas deletions at the coding regions showed that the cooperation of the two proteins expressed in Anabaena is essential for the larvicidal activity. Outdoor tests showed that the genetically altered Anabaena could keep containers with natural water from being inhabited by Culex larvae for over 2 months.
关键词[WOS]: THURINGIENSIS SUBSP ISRAELENSIS ; AGMENELLUM-QUADRUPLICATUM PR-6 ; LARVICIDAL ACTIVITY ; STRAIN PCC-7120 ; CYANOBACTERIUM ; PROTEINS ; CLONING ; LARVAE
语种: 英语
WOS记录号: WOS:000089989600009
ISSN号: 0343-8651
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/10078
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Shandong Inst Parasit Dis, Shandong 272133, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Xu, XD; Yan, G; Kong, RQ; Liu, XP; Yu, LH.Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control,CURRENT MICROBIOLOGY,2000,41(5):352-356
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