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学科主题: Plant Sciences
题名: Construction and analysis of a high-CO2-requiring mutant of the cyanobacterium Anabaena sp strain PCC7120
作者: Wu, TF; Song, LR; Liu, YD
通讯作者: Song, LR, Chinese Acad Sci, Inst Hydrobiol, Dept Phycol, Wuhan 430072, Hubei, Peoples R China
关键词: Anabaena sp strain PCC7120 ; apparent photosynthetic affinity ; carbonic anhydrase activity ; carboxysomes ; CCM mutant ; medium alkalization rate ; transposon
刊名: AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
发表日期: 2000
卷: 27, 期:11, 页:1077-1083
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Dept Phycol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
摘要: A mutant of Anabaena sp. strain PCC7120 requiring high CO2 was generated using Tn5 mutagenesis. This is the first data for a filamentous cyanobacterium. The mutant was capable of growing at 5% CO2, but incapable of growing at air levels of CO2. Southern hybridization analysis indicated that the Anabaena genome was inserted by the transposon at one site. The apparent photosynthetic affinity of the mutant to external dissolved inorganic carbon (DIC) was about 300 times lower that of the wild type (WT), and the medium alkalization rate as well as the carboxysomal carbonic anhydrase activity of the mutant was also lower than those of the WT. When the mutant was transferred from the culture medium bubbled with 5% CO2 to higher DIC (8.4% CO2) or 1% CO2, it showed similar responses to the WT. However, aberrant carboxysomes were found in the mutant cells through ultrastructural analysis, indicating it was most probably the wrong organization of the carboxysomes that eventually led to the inefficient operation of carboxysomal carbonic anhydrase and the subsequent defectiveness of the mutant in utilizing DIC.
英文摘要: A mutant of Anabaena sp. strain PCC7120 requiring high CO2 was generated using Tn5 mutagenesis. This is the first data for a filamentous cyanobacterium. The mutant was capable of growing at 5% CO2, but incapable of growing at air levels of CO2. Southern hybridization analysis indicated that the Anabaena genome was inserted by the transposon at one site. The apparent photosynthetic affinity of the mutant to external dissolved inorganic carbon (DIC) was about 300 times lower that of the wild type (WT), and the medium alkalization rate as well as the carboxysomal carbonic anhydrase activity of the mutant was also lower than those of the WT. When the mutant was transferred from the culture medium bubbled with 5% CO2 to higher DIC (8.4% CO2) or 1% CO2, it showed similar responses to the WT. However, aberrant carboxysomes were found in the mutant cells through ultrastructural analysis, indicating it was most probably the wrong organization of the carboxysomes that eventually led to the inefficient operation of carboxysomal carbonic anhydrase and the subsequent defectiveness of the mutant in utilizing DIC.
关键词[WOS]: RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE ; SYNECHOCOCCUS SP PCC-7942 ; CO2 CONCENTRATION ; INORGANIC CARBON ; PCC7942 ; GENE ; CARBOXYSOME ; REGION ; INACTIVATION ; TRANSPORT
语种: 英语
WOS记录号: WOS:000165072900010
ISSN号: 0310-7841
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/10070
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Dept Phycol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Wu, TF; Song, LR; Liu, YD.Construction and analysis of a high-CO2-requiring mutant of the cyanobacterium Anabaena sp strain PCC7120,AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY,2000,27(11):1077-1083
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