IHB OpenIR  > 期刊论文
Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: Structural characterization and effect on the complement system of polysaccharides from Nostoc commune
Lars P.Brull; Zebo Huang; Jane E.Thomas-Oates; Berit Smestad Paulsen; Ellen Hanne Cohen; Terje E.Michaelsen; Paulsen, BS, Univ Oslo, Sch Pharm, Dept Pharmacognosy, POB 1068, N-0316 Oslo, Norway
2000
Source PublicationJOURNAL OF PHYCOLOGY
Volume36Issue:5Pages:871-881
AbstractThe cyanobacterium Nostoc commune Vaucher produces quite complex extracellular polysaccharides. The cyanobacterium is nitrogen fixing, and on growing the cyanobacterium in media with and without nitrogen, different types of extracellular polysaccharides were obtained. These were also different from the polysaccharides present in N. commune collected in the field. High pH anion exchange chromatography (HPAEC) of weak acid hydrolysates of the culture-grown material demonstrated that, in this case, HPAEC was useful for comparison of the different polymers. The main differences between the polymers from the field group and the culture-grown samples were the presence of substantial amounts of arabinose, 2-O-methylglucose, and glucuronic acid in the latter. Methylation studies also revealed a difference in the branching points on the glucose units between the field and cultured samples, being 1,4,6 for the first and 1,3,6 for the latter. The field acidic fraction gave, on weak acid hydrolysis and separation on BioGel P2 and HPAEC, 12 oligosaccharide fractions that were isolated and studied by different mass spectroscopy techniques. The structures of the oligosaccharides were determined, and two different series that can originate from two repeating pentamers were identified: GlcAl-4/6GlcM1-4Ga11-4Glc1-4Xyl and GlcAl-4/6Glc1-4Ga11-4Glc1-4Xyl. The difference between these oligosaccharides lies in the methyl substituent on carbon 2 of the glucose unit next to the nonreducing glucuronic acid unit. The polysaccharides from field material were shown to have a strong effect on the complement system.
KeywordComplement Activity Cyanobacteria Hpaec 2-o-methylglucose Nostoc Commune Oligosaccharide Profiles Polysaccharides
DepartmentUniv Oslo, Sch Pharm, Dept Pharmacognosy, N-0316 Oslo, Norway; TNO, Netherlands Org Appl Sci Res, NL-3700 AJ Zeist, Netherlands; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Univ Manchester, Inst Sci & Technol, Dept Chem, Michael Barber Ctr Mass Spectrometry, Manchester M60 1QD, Lancs, England; Natl Inst Publ Hlth, Dept Vaccinol, N-0403 Oslo, Norway
Subject AreaPlant Sciences ; Marine & Freshwater Biology
Indexed BySCI
WOS IDWOS:000165409200011
Citation statistics
Cited Times:42[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10058
Collection期刊论文
Corresponding AuthorPaulsen, BS, Univ Oslo, Sch Pharm, Dept Pharmacognosy, POB 1068, N-0316 Oslo, Norway
Recommended Citation
GB/T 7714
Lars P.Brull,Zebo Huang,Jane E.Thomas-Oates,et al. Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: Structural characterization and effect on the complement system of polysaccharides from Nostoc commune[J]. JOURNAL OF PHYCOLOGY,2000,36(5):871-881.
APA Lars P.Brull.,Zebo Huang.,Jane E.Thomas-Oates.,Berit Smestad Paulsen.,Ellen Hanne Cohen.,...&Paulsen, BS, Univ Oslo, Sch Pharm, Dept Pharmacognosy, POB 1068, N-0316 Oslo, Norway.(2000).Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: Structural characterization and effect on the complement system of polysaccharides from Nostoc commune.JOURNAL OF PHYCOLOGY,36(5),871-881.
MLA Lars P.Brull,et al."Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: Structural characterization and effect on the complement system of polysaccharides from Nostoc commune".JOURNAL OF PHYCOLOGY 36.5(2000):871-881.
Files in This Item:
File Name/Size DocType Version Access License
Studies of polysacch(691KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lars P.Brull]'s Articles
[Zebo Huang]'s Articles
[Jane E.Thomas-Oates]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lars P.Brull]'s Articles
[Zebo Huang]'s Articles
[Jane E.Thomas-Oates]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lars P.Brull]'s Articles
[Zebo Huang]'s Articles
[Jane E.Thomas-Oates]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.