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学科主题: Multidisciplinary Sciences
题名: Nuclear transplantation with early-embryonic cells of transgenic fish
作者: Chen, SP ; Zhao, HB ; Sun, YH ; Zhang, FY ; Zhu, ZY
通讯作者: Chen, SP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: fish ; gene transfer ; nuclear transplantation
刊名: PROGRESS IN NATURAL SCIENCE
发表日期: 2000
卷: 10, 期:12, 页:925-930
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
摘要: Like other transgenic animals, transgenic fishes produced by microinjection are transgenic mosaics. In order to produce homogenous transgenic fish, the transgenic blastula or gastrula cells were dissociated from Carassius auratus, Pengze var, and Cyprinus carpio, Huanghe var., and the nuclei were transferred into the mature eggs of the same species via microinjection or electro-fusion. Five nuclear-transferred Carassius auratus, Pengze var. and one Cyprinus carpio, Huanghe var. were obtained and the existence of the transgene was detected. The possibility of generating homogenous strain of transgenic fish by nuclear transplantation with transgenic early-embryonic cells is discussed.
WOS记录号: WOS:000165659900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/10056
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Chen, SP; Zhao, HB; Sun, YH; Zhang, FY; Zhu, ZY.Nuclear transplantation with early-embryonic cells of transgenic fish,PROGRESS IN NATURAL SCIENCE,2000,10(12):925-930
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