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Nuclear transplantation with early-embryonic cells of transgenic fish
Chen, SP; Zhao, HB; Sun, YH; Zhang, FY; Zhu, ZY; Chen, SP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2000
Source PublicationPROGRESS IN NATURAL SCIENCE
Volume10Issue:12Pages:925-930
AbstractLike other transgenic animals, transgenic fishes produced by microinjection are transgenic mosaics. In order to produce homogenous transgenic fish, the transgenic blastula or gastrula cells were dissociated from Carassius auratus, Pengze var, and Cyprinus carpio, Huanghe var., and the nuclei were transferred into the mature eggs of the same species via microinjection or electro-fusion. Five nuclear-transferred Carassius auratus, Pengze var. and one Cyprinus carpio, Huanghe var. were obtained and the existence of the transgene was detected. The possibility of generating homogenous strain of transgenic fish by nuclear transplantation with transgenic early-embryonic cells is discussed.
KeywordFish Gene Transfer Nuclear Transplantation
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
Indexed BySCI
WOS IDWOS:000165659900006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10056
Collection期刊论文
Corresponding AuthorChen, SP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, SP,Zhao, HB,Sun, YH,et al. Nuclear transplantation with early-embryonic cells of transgenic fish[J]. PROGRESS IN NATURAL SCIENCE,2000,10(12):925-930.
APA Chen, SP,Zhao, HB,Sun, YH,Zhang, FY,Zhu, ZY,&Chen, SP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2000).Nuclear transplantation with early-embryonic cells of transgenic fish.PROGRESS IN NATURAL SCIENCE,10(12),925-930.
MLA Chen, SP,et al."Nuclear transplantation with early-embryonic cells of transgenic fish".PROGRESS IN NATURAL SCIENCE 10.12(2000):925-930.
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