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学科主题: Biology
题名: The onset of foreign gene transcription in nuclear-transferred embryos of fish
作者: Sun, YH; Chen, SP; Wang, YP; Zhu, ZY
通讯作者: Zhu, ZY, Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: transgenic fish ; nuclear transplantation ; serial nuclear transplantation ; transcription ; reverse transcriptional PCR ; reprogramming
刊名: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
发表日期: 2000-12-01
卷: 43, 期:6, 页:597-605
收录类别: SCI
文章类型: Article
部门归属: Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
摘要: The transcriptional onset of hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F-4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F-4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F-4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. it looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation.
英文摘要: The transcriptional onset of hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F-4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F-4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F-4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. it looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation.
关键词[WOS]: NUCLEOLAR ULTRASTRUCTURE ; BOVINE EMBRYO ; TRANSPLANTATION ; MATURATION ; MAMMALS
语种: 英语
WOS记录号: WOS:000166396000005
ISSN号: 1006-9305
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/10046
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
SUN Yonghua (孙永华); CHEN Shangping (陈尚萍); WANG Yaping (汪亚平) & ZHU Zuoyan (朱作言).The onset of foreign gene transcription in nuclear-transferred embryos of fish,SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2000,43(6):597-605
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