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The onset of foreign gene transcription in nuclear-transferred embryos of fish
SUN Yonghua (孙永华); CHEN Shangping (陈尚萍); WANG Yaping (汪亚平) & ZHU Zuoyan (朱作言); Zhu, ZY, Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2000
Source PublicationSCIENCE IN CHINA SERIES C-LIFE SCIENCES
Volume43Issue:6Pages:597-605
AbstractThe transcriptional onset of hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F-4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F-4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F-4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. it looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation.
KeywordTransgenic Fish Nuclear Transplantation Serial Nuclear Transplantation Transcription Reverse Transcriptional Pcr Reprogramming
DepartmentWuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiology
Indexed BySCI
WOS IDWOS:000166396000005
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10046
Collection期刊论文
Corresponding AuthorZhu, ZY, Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
SUN Yonghua ,CHEN Shangping ,WANG Yaping ,et al. The onset of foreign gene transcription in nuclear-transferred embryos of fish[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2000,43(6):597-605.
APA SUN Yonghua ,CHEN Shangping ,WANG Yaping ,&Zhu, ZY, Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2000).The onset of foreign gene transcription in nuclear-transferred embryos of fish.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,43(6),597-605.
MLA SUN Yonghua ,et al."The onset of foreign gene transcription in nuclear-transferred embryos of fish".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 43.6(2000):597-605.
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