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Transgenes in F-4 pMThGH-transgenic common carp (Cyprinus carpio L.) are highly polymorphic
ZENG Zhiqiang; ZHU Zuoyan; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2001
Source PublicationCHINESE SCIENCE BULLETIN
Volume46Issue:2Pages:143-148
AbstractTo gain information on the integration pattern of pMThGH-transgene, 50 transgenes were recovered from F-4 generation of pMThGH transgenic common carp (Cyprinus carpio L,) and 33 recovered genes were analyzed. The restriction maps of these recovered genes were constructed by digestion with five kinds of enzymes. These transgenes can be classified into 4 types according to their restriction maps. Only one type of transgenes maintains its original molecular form, whereas the other three types are very different from the original one and vary each other on both molecular weight and restriction maps. This implies that the sequences of most transgenes have been deleted and/or rearranged during integration and inheritance. The results of PCR amplification and Southern blot hybridization indicate that MThGH in Type I transgene keeps intact but most of its sequence has been deleted in other three types. All these results suggest that transgenes in F-4 generation of transgenic carp are highly polymorphic. Two DNA fragments concerning integration site of transgenes were cloned from recovered transgenes, and found to be homologous to the 5'UTR of beta -actin gene of common carp and mouse mRNA for receptor tyrosine kinase (RTK), respectively.
KeywordTransgenic Common Carp Integration Pattern Trans-gene Recovery
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
Indexed BySCI
WOS IDWOS:000167010200013
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10040
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
ZENG Zhiqiang,ZHU Zuoyan,Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. Transgenes in F-4 pMThGH-transgenic common carp (Cyprinus carpio L.) are highly polymorphic[J]. CHINESE SCIENCE BULLETIN,2001,46(2):143-148.
APA ZENG Zhiqiang,ZHU Zuoyan,&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2001).Transgenes in F-4 pMThGH-transgenic common carp (Cyprinus carpio L.) are highly polymorphic.CHINESE SCIENCE BULLETIN,46(2),143-148.
MLA ZENG Zhiqiang,et al."Transgenes in F-4 pMThGH-transgenic common carp (Cyprinus carpio L.) are highly polymorphic".CHINESE SCIENCE BULLETIN 46.2(2001):143-148.
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