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Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion
F Hongtuo & W Chingjiang; Hongtuo, F, Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China
2001
Source PublicationAQUACULTURE RESEARCH
Volume32Issue:4Pages:267-275
AbstractTo study nuclear transfer in the leach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 degreesC in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days, It was found that gastrula cells incubated at 4 degreesC had the same totipotence as blastula cells, The optimal UV dosage for inactivation of the oocyte chromatin was 180-240 mJ cm(-2). Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development.
KeywordElectrofusion Nuclear Transfer Uv Inactivation Fish Oocyte Blastomere Gastrula Cell
DepartmentChinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries
Indexed BySCI
WOS IDWOS:000168005600003
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10022
Collection期刊论文
Corresponding AuthorHongtuo, F, Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
F Hongtuo & W Chingjiang,Hongtuo, F, Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China. Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion[J]. AQUACULTURE RESEARCH,2001,32(4):267-275.
APA F Hongtuo & W Chingjiang,&Hongtuo, F, Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China.(2001).Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion.AQUACULTURE RESEARCH,32(4),267-275.
MLA F Hongtuo & W Chingjiang,et al."Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion".AQUACULTURE RESEARCH 32.4(2001):267-275.
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