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学科主题: Fisheries
题名: Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion
作者: F Hongtuo & W Chingjiang
通讯作者: Hongtuo, F, Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China
关键词: electrofusion ; nuclear transfer ; UV inactivation ; fish ; oocyte ; blastomere ; gastrula cell
刊名: AQUACULTURE RESEARCH
发表日期: 2001
卷: 32, 期:4, 页:267-275
收录类别: SCI
部门归属: Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
摘要: To study nuclear transfer in the leach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 degreesC in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days, It was found that gastrula cells incubated at 4 degreesC had the same totipotence as blastula cells, The optimal UV dosage for inactivation of the oocyte chromatin was 180-240 mJ cm(-2). Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development.
WOS记录号: WOS:000168005600003
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/10022
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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F Hongtuo & W Chingjiang.Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion,AQUACULTURE RESEARCH,2001,32(4):267-275
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