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Studies on the energy release of rice mitochondria under different conditions by means of microcalorimetry
Pei-Jiang Zhou; Han-Tao Zhou; Yi Liu; Song-Sheng Qu; Ying-Guo Zhu; Zhen-Bin Wu; Zhou, PJ, Wuhan Univ, Coll Chem & Environm Sci, Wuhan 430072, Peoples R China
2001
Source PublicationJOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Volume48Issue:1Pages:1-11
AbstractThe thermodynamic and kinetic behaviors of energy release of mitochondria isolated from rice (Oryza sative L.) were studied by using a LKB 2277 Bioactivity Monitor under different conditions. The thermogenesis curves of energy release of the rice mitochondria (which had been kept at 0-3 degreesC for 15 h and 40 day before the determination) were determined respectively at 25 and 30 degreesC, and the difference in shape of the thermogenesis curves and thermodynamic and kinetic characteristics were compared. The thermodynamic and kinetic parameters of energy release of the mitochondria in the thermogenesis increasing stage have been calculated, and the experimental thermokinetic equations of the thermogenesis have been established. The results indicated that the lower the temperature, the slower the energy release of the rice mitochondria. Both the thermogenesis and the energy release late of the rice mitochondria increased after the mitochondria was kept at lower temperature for 40 days. One can use the methods to characterize the ability of the rice mitochondria to release energy under different conditions. (C) 2001 Published by Elsevier Science B.V.
KeywordRice (Oryza Sative L.) Mitochondria Energy Release Microcalorimetry Thermokinetics
DepartmentWuhan Univ, Coll Chem & Environm Sci, Wuhan 430072, Peoples R China; Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics
Indexed BySCI
WOS IDWOS:000168139400001
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/10012
Collection期刊论文
Corresponding AuthorZhou, PJ, Wuhan Univ, Coll Chem & Environm Sci, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Pei-Jiang Zhou,Han-Tao Zhou,Yi Liu,et al. Studies on the energy release of rice mitochondria under different conditions by means of microcalorimetry[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,2001,48(1):1-11.
APA Pei-Jiang Zhou.,Han-Tao Zhou.,Yi Liu.,Song-Sheng Qu.,Ying-Guo Zhu.,...&Zhou, PJ, Wuhan Univ, Coll Chem & Environm Sci, Wuhan 430072, Peoples R China.(2001).Studies on the energy release of rice mitochondria under different conditions by means of microcalorimetry.JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,48(1),1-11.
MLA Pei-Jiang Zhou,et al."Studies on the energy release of rice mitochondria under different conditions by means of microcalorimetry".JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 48.1(2001):1-11.
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